Anti-Caspase-3 antibody [31A1067]

• 产品名称

  Anti-Caspase-3抗体[31A1067]
  参阅全部 Caspase-3 一抗

• 描述

  小鼠单克隆抗体[31A1067] to Caspase-3

• 宿主

  Mouse

• 特异性

  ab13585 recognizes an active form of Caspase 3 after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Recombinant full length protein corresponding to Human Caspase-3 aa 1-277.
  Database link: P42574

• 阳性对照

• Staurosporine-treated HeLa or Jurkat cell lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.4
  Preservative: 0.05% Sodium azide
  Constituents: 99% PBS, 0.05% BSA

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  31A1067

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab13585于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 836 Human

• Entrez Gene: 12367 Mouse

• Entrez Gene: 25402 Rat

• Omim: 600636 Human

• SwissProt: P42574 Human

• SwissProt: P70677 Mouse

• SwissProt: P55213 Rat

• Unigene: 141125 Human

• Unigene: 34405 Mouse

• Unigene: 10562 Rat

别名

• A830040C14Rik antibody

• Apopain antibody

• CASP 3 antibody

• CASP-3 antibody

• CASP3 antibody

• CASP3_HUMAN antibody

• Casp3a antibody

• Caspase 3 antibody

• Caspase 3, apoptosis-related cysteine peptidase antibody

• Caspase 3, apoptosis-related cysteine protease antibody

• Caspase 3, apoptosis-related cysteine protease a antibody

• Caspase-3 subunit p12 antibody

• Caspase3 antibody

• CC3 antibody

• CPP 32 antibody

• CPP-32 antibody

• CPP32 antibody

• CPP32B antibody

• Cysteine protease CPP32 antibody

• EC 3.4.22.56 antibody

• ICE3 antibody

• LICE antibody

• mldy antibody

• OTTHUMP00000165052 antibody

• OTTHUMP00000165053 antibody

• OTTHUMP00000165054 antibody

• PARP cleavage protease antibody

• Procaspase3 antibody

• Protein Yama antibody

• SCA 1 antibody

• SCA-1 antibody

• SCA1 antibody

• SREBP cleavage activity 1 antibody

• Yama antibody

• Yama protein antibody

• 

  Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585)

  Lane 1: Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h)
  Lane 2: Wild-type HAP1 cell lysate
  Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h)
  Lane 4: Caspase-3 knockout HAP1 cell lysate
  Lanes 1 - 4: Merged signal (red and green). Green - ab13585 observed at 32 kDa. Red - loading control, ab181602, observed at 37 kDa.
  
  ab13585 was shown to recognise pro Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (± Staurosporine treatment) were subjected to SDS-PAGE. ab13585 at a concentration of 1 µg/ml and ab8245 (loading control to GAPDH) diluted to 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

• 

  Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585)

  Western blot analysis of Caspase 3 in HeLa cells using ab13585. Cells were treated with 2 uM staurosporine for different time periods. Caspase 3 activation is detected in Western blots by the presence of Caspase 3 cleavage fragments.
  
  ab13585 detected both pro (full-length) and active (cleaved) Caspase 3, depending on the treatment time points. Pro Caspase 3 is detected at ~32 kDa. Active/cleaved Caspase 3 (large subunit) is detected at ~14-21 kDa as one or more bands.

• 

  Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585)

  All lanes : Anti-Caspase-3 antibody [31A1067] (ab13585) at 5 µg/ml
  
  Lane 1 : Human brain lysate
  Lanes 2 & 12 : Human heart lysate
  Lane 3 : Human intestine lysate
  Lane 4 : Human kidney lysate
  Lane 5 : Human liver lysate
  Lane 6 : Human lung lysate
  Lane 7 : Human muscle lysate
  Lane 8 : Human stomach lysate
  Lane 9 : Human spleen lysate
  Lane 10 : Human ovary lysate
  Lane 11 : Human testis lysate
  Lane 13 : Mouse heart lysate
  Lane 14 : Rat heart lysate
  
  Predicted band size: 31 kDa
  
  
  
  Lanes 12, 13 and 14 demonstrate the species cross-reactivity of the antibody in Human, mouse and rat heart lysate, respectively.

• 

  Western blot - Anti-Caspase-3 antibody [31A1067] (ab13585)

  All lanes : Anti-MDC1 antibody (ab13858) at 1 µg/ml
  
  Lane 1 : Hela whole cell lysate (Staurosporine treated, 2uM/4hr)
  Lane 2 : Hela whole cell lysate (untreated control)
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size: 31 kDa
  Additional bands at: 17 kDa (possible mature (processed) protein), 19 kDa (possible mature (processed) protein), 32 kDa (possible immature (unprocessed))
  
  
  
  

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab13585 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.