Anti-RAP1 antibody [4c8/1]

• 产品名称

  Anti-RAP1/TERF2IP抗体[4c8/1]
  参阅全部 RAP1/TERF2IP 一抗

• 描述

  小鼠单克隆抗体[4c8/1] to RAP1/TERF2IP

• 宿主

  Mouse

• 经测试应用

  适用于: IP, WB, Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein corresponding to Human RAP1/TERF2IP.

• 阳性对照

• In Western Blot, this antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; HepG2; A431.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 浓度

• 100 µg 浓度为 1.44 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  4c8/1

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab14404于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 54386 Human

• Omim: 605061 Human

• SwissProt: Q9NYB0 Human

• Unigene: 301419 Human

• 别名

• DRIP5 antibody

• hRap1 antibody

• MGC105533 antibody

• RAP 1 antibody

• RAP1 homolog antibody

• RAP1, yeast, homolog if antibody

• RAP1, yeast, homolog of antibody

• Repressor/activator protein 1 homolog antibody

• TE2IP_HUMAN antibody

• Telomeric Repeat Binding Factor 2 Interacting Protein antibody

• Telomeric repeat-binding factor 2-interacting protein 1 antibody

• TERF2-interacting protein antibody

• TERF2-interacting telomeric protein 1 antibody

• TERF2IP antibody

• TRF2 Interacting Telomeric Protein RAP1 antibody

• TRF2 interacting telomeric RAP1 protein antibody

• TRF2-interacting telomeric protein 1 antibody

• TRF2-interacting telomeric protein antibody

• Dopamine receptor interacting protein 5 antibody

• Dopamine receptor-interacting protein 5 antibody

• DRIP 5 antibody

• 

  Western blot - Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404)

  All lanes : Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404) at 5 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 36 kDa
  Observed band size: 55 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 8 minutes
  

• 

  Immunoprecipitation - Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404)

  RAP1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to RAP1/TERF2IP  RITM0035008 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab14404.
  Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
  Band: 55kDa; RAP1

• 

  Flow Cytometry - Anti-RAP1/TERF2IP antibody [4c8/1] (ab14404)

  Overlay histogram showing HeLa cells stained with ab14404 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14404, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.