Anti-MLH1 antibody [G168-15]

• 产品名称

  Anti-MLH1抗体[G168-15]
  参阅全部 MLH1 一抗

• 描述

  小鼠单克隆抗体[G168-15] to MLH1

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, ICC, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant full length protein.

• 阳性对照

• Tonsil, colon carcinoma. This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: HepG2

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.05% Sodium azide
  Constituent: 1% BSA

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  G168-15

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab14206于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4292 Human

• Entrez Gene: 17350 Mouse

• Omim: 120436 Human

• SwissProt: P40692 Human

• SwissProt: Q9JK91 Mouse

• Unigene: 195364 Human

• Unigene: 196006 Mouse

• 别名

• FCC 2 antibody

• FCC2 antibody

• hMLH 1 antibody

• hMLH1 antibody

• HNPCC 2 antibody

• HNPCC antibody

• HNPCC2 antibody

• MGC5172 antibody

• MLH 1 antibody

• MLH1 antibody

• MLH1_HUMAN antibody

• MutL homolog 1 (E. coli) antibody

• MutL homolog 1 antibody

• MutL homolog 1 colon cancer nonpolyposis type 2 antibody

• MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antibody

• MutL protein homolog 1 antibody

• MutL, E. coli, homolog of, 1 antibody

• COCA 2 antibody

• COCA2 antibody

• DNA mismatch repair protein Mlh1 antibody

• 

  Immunocytochemistry - Anti-MLH1 antibody [G168-15] (ab14206)

  ICC/IF image of ab14206 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab14206 at 1/50 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [G168-15] (ab14206)

  Formalin fixed paraffin embedded human tonsil stained with MLH1 using ABC and DAB chromogen.

• 

  Flow Cytometry - Anti-MLH1 antibody [G168-15] (ab14206)

  Overlay histogram showing HeLa cells stained with ab14206 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14206, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [G168-15] (ab14206)

  Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, staining MLH1 with ab14206.