Anti-Noxa antibody [114C307]

• 产品名称

  Anti-Noxa抗体[114C307]
  参阅全部 Noxa 一抗

• 描述

  小鼠单克隆抗体[114C307] to Noxa

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, WB, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Fusion protein containing human Noxa.

• 阳性对照

• WB: RL-7 cell lysate. Flow Cyt (Intra): Jurkat cells. IHC-P: Chronic Lymphocytic Leukemia (CLL) xenograft tissue.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  114C307

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13654于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5366 Human

• Entrez Gene: 58801 Mouse

• Omim: 604959 Human

• SwissProt: Q13794 Human

• SwissProt: Q9JM54 Mouse

• Unigene: 96 Human

• Unigene: 271878 Mouse

• 别名

• ATL-derived antibody

• Immediate early response protein APR antibody

• Immediate-early-response protein APR antibody

• NOXA antibody

• Phorbol 12 myristate 13 acetate induced protein 1 antibody

• Phorbol-12-myristate-13-acetate-induced protein 1 antibody

• PMA induced protein 1 antibody

• PMA-induced protein 1 antibody

• PMA-responsive gene antibody

• Pmaip1 antibody

• Protein Noxa antibody

• Adult T cell leukemia derived PMA responsive antibody

• APR antibody

• APR_HUMAN antibody

• 

  Western blot - Anti-Noxa antibody [114C307] (ab13654)

  Western blot detection of Noxa in RL-7 cell (a follicular lymphoma) lysate. A protein of approximate molecular weight of 11 kDa is detected using ab13654 at 2 µg/ml.

  Western blot detection of Noxa in RL-7 cell (a follicular lymphoma) lysate. A protein of approximate molecular weight of 11 kDa is detected using ab13654 at 2 µg/ml.

• 

  Flow Cytometry (Intracellular) - Anti-Noxa antibody [114C307] (ab13654)

  Overlay histogram showing Jurkat cells stained with ab13654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13654, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Immunohistochemistry paraffin embedded sections - Anti-Noxa antibody [114C307] (ab13654)

  Bouin-fixed, paraffin-embedded human tonsil tissue stained for Chronic Lymphocytic Leukemia (CLL) xenograft tissue stained for Noxa using ab13654 at 1/2000 dilution in immunohistochemical analysis.