Anti-NPR-C抗体
参阅全部 NPR-C 一抗
兔多克隆抗体to NPR-C
Rabbit
适用于: ICC/IFmore details
与反应: Human
预测可用于: Mouse, Cow
Synthetic peptide corresponding to Human NPR-C aa 199-212 conjugated to keyhole limpet haemocyanin.
Sequence:
QEEGLHSIYSFDET
(Peptide available as ab49406)
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituent: Whole serum
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab14355于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/800. |
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Unigene: 619466 Human
Unigene: 25259 Mouse
Entrez Gene: 4883 Human
Entrez Gene: 18162 Mouse
Omim: 108962 Human
SwissProt: P17342 Human
SwissProt: P70180 Mouse
Unigene: 237028 Human
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Immunocytochemistry/ Immunofluorescence - Anti-NPR-C antibody (ab14355)
ICC/IF image of ab14355 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14355, purified lot with known concentration equal to 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.