Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4]

• 产品名称

  Anti-c-Jun (phospho T91 + T93)抗体[C-J 4C4]
  参阅全部 c-Jun 一抗

• 描述

  小鼠单克隆抗体[C-J 4C4] to c-Jun (phospho T91 + T93)

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein corresponding to Human c-Jun (phospho T91 + T93).

• 阳性对照

• This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 浓度

• 50 µg 浓度为 1 mg/ml

• 50 µg 浓度为 1.1 mg/ml

• 纯度

  Protein A/G purified

• 克隆

  单克隆

• 克隆编号

  C-J 4C4

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13671于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3725 Human

• Omim: 165160 Human

• SwissProt: P05412 Human

• Unigene: 696684 Human

• 别名

• AP1 antibody

• cJun antibody

• Enhancer Binding Protein AP1 antibody

• Jun Activation Domain Binding Protein antibody

• JUN antibody

• Jun oncogene antibody

• JUN protein antibody

• Jun proto oncogene antibody

• JUN_HUMAN antibody

• JUNC antibody

• Oncogene JUN antibody

• p39 antibody

• Proto oncogene c jun antibody

• Proto oncogene cJun antibody

• Proto-oncogene c-jun antibody

• Transcription Factor AP 1 antibody

• Transcription factor AP-1 antibody

• Transcription Factor AP1 antibody

• V jun avian sarcoma virus 17 oncogene homolog antibody

• V jun sarcoma virus 17 oncogene homolog (avian) antibody

• V jun sarcoma virus 17 oncogene homolog antibody

• V-jun avian sarcoma virus 17 oncogene homolog antibody

• vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody

• Activator protein 1 antibody

• AP 1 antibody

• AP-1 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] (ab13671)

  ICC/IF image of ab13617 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13617, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] (ab13671)

  IHC image of c-Jun (phospho T91 + T93) staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.