Anti-Mus81抗体[MTA30 2G10/3]
参阅全部 Mus81 一抗
小鼠单克隆抗体[MTA30 2G10/3] to Mus81
Mouse
适用于: WB, Flow Cytmore details
与反应: Human
不与反应: Mouse
Mus81 protein.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 1 mg/ml
Protein A purified
from TCS
单克隆
MTA30 2G10/3
Sp2/0-Ag14
IgG2a
Abpromise™承诺保证使用ab14387于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (7) | 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract. |
| Flow Cyt | Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Entrez Gene: 80198 Human
Omim: 606591 Human
SwissProt: Q96NY9 Human
Unigene: 288798 Human
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![Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)](https://www.abcam.cn/ps/products/14/ab14387/Images/ab14387-21896-anti-mus81-antibody-mta30-2g10-3-western-blot.jpg)
Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate
Predicted band size: 66 kDa
Observed band size: 64 kDawhy is the actual band size different from the predicted?
![Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)](https://www.abcam.cn/ps/products/14/ab14387/Images/ab14387-21895-anti-mus81-antibody-mta30-2g10-3-flow-cytometry.jpg)
Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
