Anti-TAGLN/Transgelin抗体
参阅全部 TAGLN/Transgelin 一抗
兔多克隆抗体to TAGLN/Transgelin
Rabbit
The immunogen used to generate this TAGLN/Transgelin antibody (ab14106) shares 75% homology with family members TAGLN2 and TAGLN3. Cross-reactivity with these proteins has not been confirmed experimentally.
适用于: ICC/IF, WBmore details
与反应: Mouse, Rat, Human
Synthetic peptide within Mouse TAGLN/Transgelin aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available as ab16067)
WB: Human primary smooth muscle cells; HeLa whole cell lysate; Mouse colon tissue lysate; Rat colon tissue lysate. ICC/IF: HeLa cells; mouse muscle cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.9 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab14106于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (8) | Use a concentration of 1 - 5 µg/ml. |
| WB | (12) | Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
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Unigene: 34397 Rat
Entrez Gene: 6876 Human
Entrez Gene: 21345 Mouse
SwissProt: Q01995 Human
SwissProt: P37804 Mouse
Unigene: 410977 Human
Unigene: 283283 Mouse
SM22-alpha antibody
Smooth muscle protein 22-alpha antibody
TAGL_HUMAN antibody
TAGLN antibody
Transgelin antibody
WS3-10 antibody
22 kDa actin-binding protein antibody
Protein WS3-10 antibody
SM22 antibody
Western blot - Anti-TAGLN/Transgelin antibody (ab14106)This image is a courtesy of Anonymous Abreview
All lanes : Anti-TAGLN/Transgelin antibody (ab14106) at 1/1000 dilution
All lanes : Lysates prepared from human primary smooth muscle cells
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated bovine polyclonal to rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 2 minutes
Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (ab14106)
ab14106 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol for 5 minutes, permeabilized in 0.1% PBS-Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour at room temperature to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14106 at 1 µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature.
DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence - Anti-TAGLN/Transgelin antibody (ab14106)This image is courtesy of an anonymous Abreview.
ab14106 staining SM22 alpha in mouse muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/1000.
Western blot - Anti-TAGLN/Transgelin antibody (ab14106)
All lanes : Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa nuclear extract
Lane 3 : Human skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
The band was completed abolished by peptide blocking with ab16067 (immunizing peptide) - not shown.
Western blot - Anti-TAGLN/Transgelin antibody (ab14106)
All lanes : Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/ml
Lane 1 : Human colon tissue lysate - total protein (ab30051)
Lane 2 : Mouse colon tissue lysate
Lane 3 : Rat colon tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 115 kDa, 20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 seconds
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab14106 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Western blot - Anti-TAGLN/Transgelin antibody (ab14106)
Anti-TAGLN/Transgelin antibody (ab14106) at 1 µg/ml + Recombinant Human TAGLN/Transgelin protein (ab101469) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 30 seconds
