Anti-Psoriasin antibody [47C1068]

• 产品名称

  Anti-Psoriasin抗体[47C1068]
  参阅全部 Psoriasin 一抗

• 描述

  小鼠单克隆抗体[47C1068] to Psoriasin

• 宿主

  Mouse

• 经测试应用

  适用于: IP, ICC/IF, Flow Cyt, IHC-Fr, Flow Cyt (Intra), WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein corresponding to Human Psoriasin.
  Database link: P31151

• 阳性对照

• Confluent MCF10A cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 0.05% BSA

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  This antibody is affinity purified.

• 克隆

  单克隆

• 克隆编号

  47C1068

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab13680于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Western blot - Anti-Psoriasin antibody [47C1068] (ab13680)

  All lanes : Anti-Psoriasin antibody [47C1068] (ab13680) at 1 µg/ml
  
  Lane 1 : MCF10A cell lysate
  Lane 2 : MCF7 cell lysate
  
  Predicted band size: 12.1 kDa
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [47C1068] (ab13680)

  Immunohistochemistry analysis of human esophageal squamous cell carcinoma (SCC) staining psoriasin with ab13680 in 5 µg/mL concentration.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Psoriasin antibody [47C1068] (ab13680)

  Immunohistochemistry analysis of formalin-fixed paraffin-embedded human skin tissue after heat-induced antigen retrieval, staining psoriasin with ab13680 in 10 µg/mL concentration.

• 

  Western blot - Anti-Psoriasin antibody [47C1068] (ab13680)

  Western blot analysis of Psoriasin in confluent MCF10A cells using ab13680 at 1 µg/ml.

  Western blot analysis of Psoriasin in confluent MCF10A cells using ab13680 at 1 µg/ml.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Psoriasin antibody [47C1068] (ab13680)

  ICC/IF image of ab13680 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13680, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.