HA tag peptide

• 产品名称

  HA tag多肽

• 纯度

  > 90 % HPLC.
  
  

• Accession

  P03437

• 无动物成分

  Yes

• 性质

  Synthetic

Our Abpromise guarantee covers the use of ab13835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  Blocking - Blocking peptide for Anti-HA tag antibody (ab13834)

• 形式

  Lyophilized

• 补充说明

  - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
  - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
  - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
  - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
  - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

• 稳定性和存储

  Shipped at 4°C. Store at -20°C.

  Information available upon request.

别名

• HA epitope tag

• HA1

• HA2

• hemagglutinin

• Hemagglutinin HA1 chain

• Hemagglutinin HA2 chain

• 

  Western blot - HA tag peptide (ab13835)

  Western blot using ab13834 on 293 cells transfected with a 55 kDa HA-tagged minigene (ab20896). Lane 1: ab13834 Lane 2: ab13834 with blocking peptide ab13835. Primary: Rabbit polyclonal to HA tag (1/500) Secondary: Alexa Fluor 680 Goat anti Rabbit IgG (1/5000). Lysates at 10µg/lane.