Anti-Aurora A antibody [35C1]

• 产品名称

  Anti-Aurora A抗体[35C1]
  参阅全部 Aurora A 一抗

• 描述

  小鼠单克隆抗体[35C1] to Aurora A

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt (Intra), WB, ICC/IFmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse

• 免疫原

  Recombinant full length protein corresponding to Human Aurora A.

• 阳性对照

• Human HeLa and mouse M-ICc12 cell lysates for Western blotting and human 293 or mouse LLC1 cell lines for IF. Flow Cyt (Intra): HeLa cells. ICC: HeLa cells

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  35C1

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab13824于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 6790 Human

• Entrez Gene: 20878 Mouse

• Omim: 603072 Human

• SwissProt: O14965 Human

• SwissProt: P97477 Mouse

• Unigene: 250822 Human

• Unigene: 249363 Mouse

• 别名

• AURA antibody

• Aurka antibody

• Aurora 2 antibody

• Aurora A antibody

• Aurora kinase A antibody

• Aurora-related kinase 1 antibody

• Aurora/IPL1 like kinase antibody

• AURORA/IPL1-like kinase antibody

• Aurora/IPL1-related kinase 1 antibody

• AURORA2 antibody

• Breast tumor-amplified kinase antibody

• BTAK antibody

• hARK1 antibody

• IAK antibody

• IPL1 related kinase antibody

• MGC34538 antibody

• OTTHUMP00000031340 antibody

• OTTHUMP00000031341 antibody

• OTTHUMP00000031342 antibody

• OTTHUMP00000031343 antibody

• OTTHUMP00000031344 antibody

• OTTHUMP00000031345 antibody

• OTTHUMP00000166071 antibody

• OTTHUMP00000166072 antibody

• PPP1R47 antibody

• Protein phosphatase 1, regulatory subunit 47 antibody

• Serine/threonine kinase 15 antibody

• Serine/threonine kinase 6 antibody

• Serine/threonine protein kinase 15 antibody

• Serine/threonine-protein kinase 15 antibody

• Serine/threonine-protein kinase 6 antibody

• Serine/threonine-protein kinase aurora-A antibody

• STK15 antibody

• STK6 antibody

• STK6_HUMAN antibody

• STK7 antibody

• AIK antibody

• ARK-1 antibody

• ARK1 antibody

• 

  Western blot - Anti-Aurora A antibody [35C1] (ab13824)

  All lanes : Anti-Aurora A antibody [35C1] (ab13824) at 5 µg/ml
  
  Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 46 kDa
  Observed band size: 50 kDawhy is the actual band size different from the predicted?
  Additional bands at: 125 kDa (possible non-specific binding), 55 kDa (possible non-specific binding)
  
  
  Exposure time: 20 minutes
  
  
  

  This blot was produced using 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200v for 50 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab13824 over night at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Aurora A antibody [35C1] (ab13824)

  This data was developed using the same antibody clone in a different buffer formulation without PBS and sodium azide (ab264552)

  ab264552 staining Aurora A in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab264552 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Aurora A antibody [35C1] (ab13824)This image is courtesy of an Abreview submitted by Dr Kirk McManus

  ab13824, at 1/2000 dilution, detecting Aurora A (green) in Hela Cells in conjunction with a Goat anti-mouse secondary antibody conjugated to Cy3®. Cells were fixed with methanol and counterstained with DAPI. Please refer to abreview for further details.

  See Abreview

• 

  Flow Cytometry (Intracellular) - Anti-Aurora A antibody [35C1] (ab13824)

  Overlay histogram showing HeLa cells stained with ab13824 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13824, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.