Anti-NPR-A抗体
兔多克隆抗体to NPR-A
Rabbit
适用于: ICC, IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WBmore details
与反应: Rat, Human
Synthetic peptide corresponding to Human NPR-A aa 294-308 conjugated to keyhole limpet haemocyanin.
Sequence:
LKQLKHLAYEQFNFT
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab14356于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC | Use at an assay dependent concentration. | |
IHC-Fr | Use at an assay dependent concentration. | |
IHC-P | (1) | Use at an assay dependent concentration. |
ICC/IF | 1/300. | |
Flow Cyt | (1) | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB | (1) | Use at an assay dependent concentration. |
Entrez Gene: 4881 Human
Omim: 108960 Human
SwissProt: P16066 Human
Unigene: 490330 Human
Unigene: 10463 Rat
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Immunocytochemistry/ Immunofluorescence - Anti-NPR-A antibody (ab14356)
ICC/IF image of ab14356 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14356, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.