Annexin V-Cy5 Apoptosis Staining / Detection Kit

• 产品名称

  Annexin V-Cy5 Apoptosis Staining / Detection试剂盒
  参阅全部 Annexin V/ANXA5 试剂盒

• 样品类型

  Adherent cells, Suspension cells

• 检测类型

  Direct

• 检测时间

  0h 10m

• 产品概述

  Annexin V-Cy5 Apoptosis Staining / Detection Kit ab14150 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane.

  
  

  Fluorescence can be detected at Ex/Em = 649/670 nm.

  
  

  Analysis is by flow cytometry or fluorescence microscopy.

  
  

  The Annexin V-Cy5 reagent contained in the kit is also available as Annexin V-Cy5 reagent ab14147.

• 说明

  This product is manufactured by BioVision, an Abcam company and was previously called K103 Annexin V-Cy5 Apoptosis Kit. K103-100 is the same size as the 100 test size of ab14150.

  Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.

  For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

  
  

  The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.

• 平台

  Flow cytometer, Fluorescence microscope

• 存放说明

  Store at +4°C. Please refer to protocols.

• 组件	100 tests
  Annexin V-Cy5	1 x 500µl
  Binding Buffer II	1 x 50ml

别名

• Anchorin CII

• Annexin 5

• Annexin A5

• Annexin V

• Annexin-5

• Annexin5

• AnnexinA5

• AnnexinV

• ANX 5

• ANX A5

• ANX5

• ANXA5

• ANXA5_HUMAN

• Calphobindin I

• CBP-I

• Endonexin II

• ENX 2

• ENX2

• Lipocortin V

• PAP I

• PAP-I

• Placental anticoagulant protein 4

• Placental anticoagulant protein I

• PLACENTAL PROTEIN 4

• PP 4

• Pp4

• RPRGL3

• Thromboplastin inhibitor

• VAC-alpha

• Vascular anticoagulant-alpha

• 

  Impact of different Rep constructs on DNA damage response and apoptosis - ab14150Image from Seyffert M et al., PLoS One 12(1), fig 3d. Doi: 10.1371/journal.pone.0170908. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Vero cells were co-transfected with different Rep-constructs together with a plasmid expressing eGFP to identify successful transfection. After three days, cells were stained using Annexin V-Cy5 Apoptosis staining/detection kit (ab14150) and analyzed by flow cytometry with filters for eGFP and Cy5.