Anti-beta Catenin抗体
参阅全部 beta Catenin 一抗
兔多克隆抗体to beta Catenin
Rabbit
Reacts in dot blot with beta-catenin peptide 768-781 conjugated to BSA. In immunoblots, reacts with a 94kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cells. Specific staining is inhibited following pre-incubation of the antiserum with the beta-catenin peptide. Shows no reactivity with BSA conjugated alpha catenin peptide (amino acids 890-901). The antibody does not cross react with a-catenin or ?-catenin (plakoglobin).
适用于: ICC/IF, WB, IHC-Pmore details
与反应: Rat, Cow, Human
Synthetic peptide:
PGDSNQLAWFDTDL
conjugated to KLH, corresponding to amino acids 768-781 of Human or mouse ß Catenin. (Peptide available as ab16377)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.097% Sodium azide
Constituent: Whole serum
Whole antiserum
Delipidized antiserum.
多克隆
IgG
Abpromise™承诺保证使用ab6302于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (8) | 1/2000. |
| WB | (8) | 1/4000. Predicted molecular weight: 85 kDa. |
| IHC-P | (4) | Use at an assay dependent concentration. |
Entrez Gene: 1499 Human
Omim: 116806 Human
SwissProt: P35222 Human
Unigene: 476018 Human
Unigene: 112601 Rat
b-catenin antibody
Beta catenin antibody
Beta-catenin antibody
Cadherin associated protein antibody
Catenin (cadherin associated protein), beta 1, 88kDa antibody
Catenin beta 1 antibody
Catenin beta-1 antibody
CATNB antibody
CHBCAT antibody
CTNB1_HUMAN antibody
CTNNB antibody
CTNNB1 antibody
DKFZp686D02253 antibody
FLJ25606 antibody
FLJ37923 antibody
OTTHUMP00000162082 antibody
OTTHUMP00000165222 antibody
OTTHUMP00000165223 antibody
OTTHUMP00000209288 antibody
OTTHUMP00000209289 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling beta Catenin with ab6302 at 1/20,000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab6302)
Immunocytochemistry analysis of bovine kidney cells labeling beta Catenin with ab6302 at 1/20,000 dilution. Cells were fixed and permeabilized with Methanol followed by Acetone. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat. Cells were counterstained with DAPI (blue) to stain nuclei.
Western blot - Anti-beta Catenin antibody (ab6302)
Anti-beta Catenin antibody (ab6302) at 1/8000 dilution + Bovine kidney tissue lysate
Predicted band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling beta Catenin with ab6302 at 1/20,000 dilution.
Western blot - Anti-beta Catenin antibody (ab6302)
All lanes : Anti-beta Catenin antibody (ab6302) at 1/4000 dilution
Lane 1 : 5ug human lung tumour lysate.
Lane 2 : 10ug human lung tumour lysate.
Lane 3 : 20ug human lung tumour lysate.
Secondary
All lanes : Goat anti-Rabbit IgG (H&L)HRP
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 95 kDawhy is the actual band size different from the predicted?
Exposure time: 10 seconds
This image is courtesy of an Abreview submitted by Mike Campa on 4 April 2006.
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab6302)This image is courtesy of an Abreview submitted by Jennifer Schnabel
ab6302 at 1/2000 dilution staining beta Catenin in human HeLa cells by immunocytochemistry/ immunofluorescence. Sections were paraformaldehyde fixed, permeabilized in 0.3% Triton X prior to blocking in 5% BSA for 2 hours at 27°C and then incubated with ab6302 for 8 hour at 4°C. Alexa fluor® 488 goat polyclonal, diluted 1/1000, was used as the secondary antibody. Counterstaining with DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab6302)This image is courtesy of an Abreview submitted by Roderick Benson
ab6302 at 1/500 dilution staining beta Catenin in human breast cells by immunocytochemistry/ immunofluorescence. Sections were formaldehyde fixed, permeabilized in 0.5% digitonin prior to incubating with ab6302 for 1 hour. Alexa fluor® 488 goat polyclonal, diluted 1/500, was used as the secondary antibody. Treated samples recieved 10um GSK-3 Inhibitor X.
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab6302)
This picture was kindly supplied as part of the review submitted by Mohaiza Dashwood. Immunofluorescence of H29 cells stained with DAPI (blue) and rabbit polyclonal anti-beta-catenin (ab6302), 1/2000) with Alexa Fluor 488 (green) from Molecular Probes.
