Anti-CD44 antibody [F10-44-2]

• 产品名称

  Anti-CD44抗体[F10-44-2]
  参阅全部 CD44 一抗

• 描述

  小鼠单克隆抗体[F10-44-2] to CD44

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, ICC/IF, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• In Flow Cytometry, this antibody gave a positive signal in peripheral blood lymphocytes. In IHC, this antibody gave a positive signal in human kidney carcinoma sections. ICC/IF: A431 cell line.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  F10-44-2

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab6124于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 960 Human

• Omim: 107269 Human

• SwissProt: P16070 Human

• Unigene: 502328 Human

• 别名

• CD44 antibody

• CD44 antigen antibody

• CD44 molecule (Indian blood group) antibody

• CD44 molecule antibody

• CD44_HUMAN antibody

• CDw44 antibody

• CDW44 antigen antibody

• Cell surface glycoprotein CD44 antibody

• chondroitin sulfate proteoglycan 8 antibody

• CSPG8 antibody

• ECMR-III antibody

• Epican antibody

• Extracellular matrix receptor III antibody

• GP90 lymphocyte homing/adhesion receptor antibody

• HCELL antibody

• hematopoietic cell E- and L-selectin ligand antibody

• Heparan sulfate proteoglycan antibody

• Hermes antigen antibody

• homing function and Indian blood group system antibody

• HSA antibody

• HUTCH-I antibody

• HUTCH1 antibody

• HUTCHI antibody

• Hyaluronate receptor antibody

• IN antibody

• INLU-related p80 Glycoprotein antibody

• MC56 antibody

• MDU2 antibody

• MDU3 antibody

• MGC10468 antibody

• MIC4 antibody

• MUTCH I antibody

• MUTCH1 antibody

• PGP-1 antibody

• PGP-I antibody

• PGP1 antibody

• Phagocytic glycoprotein 1 antibody

• Phagocytic glycoprotein I antibody

• Soluble CD44 antibody

• LHR antibody

• BA-1 antibody

• CD 44 antibody

• 

  Flow Cytometry - Anti-CD44 antibody [F10-44-2] (ab6124)

  Overlay histogram showing peripheral blood lymphocytes stained with ab6124 (red line). The cells were incubated with the antibody (ab6124, 0.5µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)Image from Le Bras GF et al., PLoS One. 2011;6(11):e27063. Epub 2011 Nov 1. Fig 3.; doi:10.1371/journal.pone.0027063; November 1, 2011, PLoS ONE 6(11): e27063.

  Immunohistochemical analysis of Human esophogeal keratinocytes, staining CD44 with ab6124.
  
  Keratinocytes were cultured in vivo to form an epithelium for 15 days before fixing in formaldehyde and embedding in paraffin. Primary antibody was incubated overnight at 4°C and secondary antibody for 30 minutes at 37°C. Staining was detected using DAB.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [F10-44-2] (ab6124)

  ab6124 staining CD44 in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6124 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)

  IHC image of CD44 staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6124, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.