Anti-M6PR (cation independent) antibody [2G11]

• 产品名称

  Anti-M6PR (cation independent)抗体[2G11]
  参阅全部 M6PR (cation independent) 一抗

• 描述

  小鼠单克隆抗体[2G11] to M6PR (cation independent)

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, Flow Cyt (Intra)more details
  不适用于: WB

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Cow, Non human primates, African green monkey不与反应: Hamster

• 免疫原

  Full length protein. This information is proprietary to Abcam and/or its suppliers.

• 表位

  This antibody is shown to recognize an epitope in the extracellular domain of Mannose 6 Phosphate Receptor.

• 阳性对照

• Flow Cyt (Intra): A431 cells. ICC/IF: HAP1 cells (HAP1-IGF2R knockout cells used as negative cell line).

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 纯度

  Ammonium Sulphate Precipitation

• 克隆

  单克隆

• 克隆编号

  2G11

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab2733于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for WB.

数据库链接

• Entrez Gene: 281849 Cow

• Entrez Gene: 3482 Human

• Entrez Gene: 16004 Mouse

• Entrez Gene: 25151 Rat

• Omim: 147280 Human

• SwissProt: P08169 Cow

• SwissProt: P11717 Human

• SwissProt: Q07113 Mouse

• Unigene: 487062 Human

• Unigene: 673278 Human

• Unigene: 26553 Mouse

• Unigene: 486511 Mouse

别名

• 300 kDa mannose 6 phosphate receptor antibody

• 300 kDa mannose 6-phosphate receptor antibody

• Cation independent mannose 6 phosphate receptor antibody

• Cation-independent mannose-6-phosphate receptor antibody

• CD222 antibody

• CD222 antigen antibody

• CI Man 6 P receptor antibody

• CI Man-6-P receptor antibody

• CI MPR antibody

• CI-M6PR antibody

• CI-MPR antibody

• CIMPR antibody

• IGF 2 receptor antibody

• IGF 2R antibody

• IGF II receptor antibody

• IGF-II receptor antibody

• IGF2 receptor antibody

• Igf2r antibody

• Insulin like growth factor 2 receptor antibody

• Insulin like growth factor II receptor antibody

• Insulin-like growth factor 2 receptor antibody

• Insulin-like growth factor II receptor antibody

• M6P R antibody

• M6P/IGF2 receptor antibody

• M6P/IGF2R antibody

• M6PR antibody

• mannose 6 phosphate receptor antibody

• mannose 6 phosphate receptor, cation independent antibody

• MPR 300 antibody

• MPR300 antibody

• MPRI antibody

• MPRI_HUMAN antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

  ab2733 staining IGF2R in wild-type HAP1 cells (top panel) and IGF2R knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab2733 at 1ug/ml and ab6046 (Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in pseudo color red).  Nuclear DNA was labelled in blue with DAPI.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

  ICC/IF image of ab2733 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2733, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

• 

  Flow Cytometry (Intracellular) - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

  Overlay histogram showing HeLa cells stained with ab2733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2733, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733)Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK

  Immunofluorescent imaging of human cells (U2OS) with ab2733 confirms the specificity of this antibody, with the expected perinuclear vesicular staining of lysosomes. 

  IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

  ab2733 positively staining formaldehyde fixed Human HEK 293 cells (red) in conjunction with goat anti mouse (Alexa 546). Nuclear staining was obtained using Hoechst.
  This image is an edited version of an image received courtesy of an Abreview submitted by Kun Liu on 19 September 2005. We do not have any further information relating to this image.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody [2G11] (ab2733)

  ICC/IF image of ab2733 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2733, 10µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.