Anti-Prolactin Receptor/PRL-R antibody [U5]

• 产品名称

  Anti-Prolactin Receptor/PRL-R抗体[U5]
  参阅全部 Prolactin Receptor/PRL-R 一抗

• 描述

  小鼠单克隆抗体[U5] to Prolactin Receptor/PRL-R

• 宿主

  Mouse

• 特异性

  Detects prolactin (PRL) receptor.

• 经测试应用

  适用于: Flow Cyt, IHC-P, ICC/IF, IP, Inhibition Assay, IHC-Fr, WBmore details

• 种属反应性

  与反应: Rat, Sheep, Rabbit, Horse, Human, Pig
  预测可用于: Non human primates

• 免疫原

  Full length native protein (purified) corresponding to Rat Prolactin Receptor/PRL-R. Purified rat liver PRL receptor.

• 表位

  This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  U5

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2772于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5618 Human

• Entrez Gene: 24684 Rat

• Omim: 176761 Human

• SwissProt: P16471 Human

• SwissProt: P05710 Rat

• Unigene: 368587 Human

• Unigene: 9757 Rat

• 别名

• Delta 4-delta 7/11 truncated prolactin receptor antibody

• Delta 4-SF1b truncated prolactin receptor antibody

• HPRL antibody

• hPRL receptor antibody

• hPRLrI antibody

• Lactogen receptor antibody

• MFAB antibody

• MGC105486 antibody

• OPR antibody

• OTTHUMP00000115998 antibody

• Pr-1 antibody

• Pr-3 antibody

• PRL R antibody

• PRL-R antibody

• PRLR antibody

• Prlr-rs1 antibody

• PRLR_HUMAN antibody

• Prolactin receptor a antibody

• Prolactin receptor antibody

• Prolactin receptor delta 7/11 antibody

• RATPRLR antibody

• Secreted prolactin binding protein antibody

• Truncated testis-specific box 1-C prolactin receptor antibody

• wu:fj65c07 antibody

• AI987712 antibody

• CLONE SPM213 antibody

• CPRLP antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [U5] (ab2772)

  Mammary gland tissue from lactating light horse mare was incubated with ab2772 at 1:100 for 1 hour at 23°C and for 24 hours at 4°C. A biotin-conjugated goat anti-mouse IgG was used as the secondary antibody (23°C for 1 hour) .Development using avidin-biotin complex 23°C for 1 hour plus 3,3-diaminobenzadine for 15 min and counterstained with haematoxylin. Antigen retrieval was facilitated using steaming at 95°C for 20 min in Trizma® buffer (pH 10.0) . Blocking endogenous peroxidase performed with 0.3% H2O2 and MeOH (20 min at 23°C). To decrease non-specific staining, tissue was pre-incubated in normal goat serum for 30 min at 23°C.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [U5] (ab2772)

  Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor/PRL-R shows staining in A2058 cells. Prolactin Receptor/PRL-R (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2772 (1:20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Flow Cytometry - Anti-Prolactin Receptor/PRL-R antibody [U5] (ab2772)

  Overlay histogram showing MCF-7 cells stained with ab2772 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2772, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [U5] (ab2772)

  Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor/PRL-R shows staining in HepG2 cells. Prolactin Receptor/PRL-R (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2772 (1:20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor/PRL-R antibody [U5] (ab2772)

  Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor/PRL-R shows staining in MCF-7 cells. Prolactin Receptor/PRL-R (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2772 (1:20) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor/PRL-R antibody [U5] (ab2772)This image is courtesy of an anonymous Abreview

  ab2772 staining the Prolactin Receptor/PRL-R in Human salivary gland tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.

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