Anti-SERCA2 ATPase antibody [IID8]

产品名称

 Anti-SERCA2 ATPase抗体[IID8]

参阅全部 SERCA2 ATPase 一抗

• 描述

  小鼠单克隆抗体[IID8] to SERCA2 ATPase

• 宿主

  Mouse

• 特异性

  Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. This antibody does not discriminate between the two isoforms. By Western blot, this antibody detects an ~110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Immunofluorescence staining of SECRA2 ATPase in rabbit skeletal muscle results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization. This antibody is not recommended for Western blot detection of rat SERCA2.

• 经测试应用

  适用于: WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• IHC-P: Human skeletal muscle and cardiac muscle tissue. WB: Human skeletal muscle. HeLa, HepG2, A549 and A673 whole cell lysate.

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 25th November 2020.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 浓度

• 批次浓度范围 100 µg 浓度为 0.532 - 0.992 mg/ml

• 1 mg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• Primary antibody说明

  ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

• 克隆

  单克隆

• 克隆编号

  IID8

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2817于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 488 Human

• Omim: 108740 Human

• SwissProt: P16615 Human

• Unigene: 506759 Human

• 别名

• ATPase Ca++ transporting cardiac muscle slow twitch 2 antibody

• Calcium pump 2 antibody

• Calcium-transporting ATPase sarcoplasmic reticulum type antibody

• Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform antibody

• Cardiac Ca2+ ATPase antibody

• DAR antibody

• DD antibody

• Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody

• MGC45367 antibody

• Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 antibody

• SERCA 2 antibody

• SERCA2 antibody

• serca2a antibody

• slow twitch skeletal muscle isoform antibody

• SR Ca(2+)-ATPase 2 antibody

• AT2A2_HUMAN antibody

• Atp2a2 antibody

• ATP2B antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [IID8] (ab2817)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SERCA2 ATPase with ab2817 at 1/4000 dilution, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human skeletal muscle tissue is observed. Counterstained with hematoxylin.
  Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
  Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [IID8] (ab2817)

  Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling SERCA2 ATPase with ab2817 at 1/4000 dilution, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cardiac muscle tissue is observed. Counterstained with hematoxylin.
  Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
  Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Western blot - Anti-SERCA2 ATPase antibody [IID8] (ab2817)

  All lanes : Anti-SERCA2 ATPase antibody [IID8] (ab2817) at 1/1000 dilution
  
  Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
  Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
  Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
  Lane 4 : A673 (human muscle Ewing's Sarcoma), whole cell lysate
  Lane 5 : Human skeletal muscle
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution
  
  Observed band size: 110 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

  Exposure times: Lanes 1-2: 92 seconds; Lanes 3-4: 10 seconds; Lane 5: 3 seconds.

  Samples are non-boiled as boiling may cause protein aggregates.