Anti-Cytohesin 2 antibody [10A12]

• 产品名称

  Anti-Cytohesin 2抗体[10A12]
  参阅全部 Cytohesin 2 一抗

• 描述

  小鼠单克隆抗体[10A12] to Cytohesin 2

• 宿主

  Mouse

• 特异性

  Detects recombinant human cytohesin-2 (ARNO).

• 经测试应用

  适用于: ICC/IF, IHC-Pmore details
  不适用于: WB

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant full length protein (His-tag) corresponding to Human Cytohesin 2.
  Database link: Q99418

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 纯化说明

  PEG purified IgG1

• 克隆

  单克隆

• 克隆编号

  10A12

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2728于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for WB.

• 数据库链接

• Entrez Gene: 9266 Human

• Entrez Gene: 19158 Mouse

• Omim: 602488 Human

• SwissProt: Q99418 Human

• SwissProt: P63034 Mouse

• Unigene: 144011 Human

• Unigene: 272130 Mouse

• 别名

• Arno antibody

• ARNO protein antibody

• CLM2 antibody

• CTS18 antibody

• CTS18.1 antibody

• CYH2_HUMAN antibody

• Cyth2 antibody

• Cytohesin 2 antibody

• Cytohesin-2 antibody

• MGC137537 antibody

• MGC80440 antibody

• PH antibody

• PH, SEC7 and coiled-coil domain-containing protein 2 antibody

• Pleckstrin homology Sec7 and coiled coil domains 2 antibody

• Pleckstrin homology Sec7 and coiled coil domains protein 2 antibody

• Protein ARNO antibody

• PSCD2 antibody

• PSCD2L antibody

• PSCD2L, formerly antibody

• SEC7 and coiled-coil domain-containing protein 2 antibody

• Sec7 antibody

• SEC7 homolog B antibody

• Sec7B antibody

• SEC7L antibody

• Sec7p L antibody

• Sec7p-like antibody

• Sec7pL antibody

• ARF exchange factor antibody

• ARF nucleotide binding site opener antibody

• ARF nucleotide-binding site opener antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)

  Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)

  Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (ab2728)

  Immunocytochemistry/Immunofluorescence analysis of Cytohesin 2 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2728 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human colon carcinoma tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 2 antibody [10A12] (ab2728)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human stomach tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2728 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.