Anti-Aly/Ref antibody [11G5]

• 产品名称

  Anti-Aly/Ref抗体[11G5]
  参阅全部 Aly/Ref 一抗

• 描述

  小鼠单克隆抗体[11G5] to Aly/Ref

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, IHC-P, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein corresponding to Human Aly/Ref.
  Database link: Q86V81

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from supernatant.

• 克隆

  单克隆

• 克隆编号

  11G5

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab6141于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 10189 Human

• Omim: 604171 Human

• SwissProt: Q86V81 Human

• Unigene: 534385 Human

• 别名

• ALY/REF antibody

• Aly/REF export factor antibody

• BEF antibody

• bZIP enhancing factor antibody

• bZIP-enhancing factor BEF antibody

• REF antibody

• THO complex 4 antibody

• THO complex subunit 4 antibody

• Tho4 antibody

• thoc4 antibody

• THOC4_HUMAN antibody

• Transcriptional coactivator Aly/REF antibody

• Transcriptional coactivator antibody

• Ally of AML-1 and LEF-1 antibody

• Ally of AML1 and LEF1 antibody

• ALY antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Aly/Ref antibody [11G5] (ab6141)

  ICC/IF image of ab6141 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6141, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aly/Ref antibody [11G5] (ab6141)

  IHC image of ab6141 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6141, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

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  Flow Cytometry - Anti-Aly/Ref antibody [11G5] (ab6141)

  Overlay histogram showing HeLa cells stained with ab6141 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6141, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aly/Ref antibody [11G5] (ab6141)

  Immunohistochemistry showing Aly/Ref in formalin fixed paraffin embedded skin tissue using ab6141 at 1 μg/ml.