Anti-Glycogen synthase 1/GYS1 (phospho S641)抗体
参阅全部 Glycogen synthase 1/GYS1 一抗
兔多克隆抗体to Glycogen synthase 1/GYS1 (phospho S641)
Rabbit
Phospho Glycogen Synthase pS641 antibody is directed against human muscle glycogen synthase. This phospho specific polyclonal antibody is specific for phosphorylated pS641 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.
适用于: IHC-P, WBmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Human Glycogen synthase 1/GYS1 aa 600-700 (phospho S641) conjugated to keyhole limpet haemocyanin.
Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Phospho Glycogen Synthase pS641 antibody is directed against human muscle glycogen synthase. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.
多克隆
IgG
Abpromise™承诺保证使用ab2479于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. |
Entrez Gene: 2997 Human
Entrez Gene: 14936 Mouse
Omim: 138570 Human
SwissProt: P13807 Human
SwissProt: Q9Z1E4 Mouse
Unigene: 386225 Human
Unigene: 275654 Mouse
GSY antibody
GYS antibody
Gys1 antibody
GYS1_HUMAN antibody
muscle antibody
Glycogen [starch] synthase antibody
Glycogen synthase 1 (muscle) antibody
Glycogen synthase 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479)
Immunohistochemistry with Anti-Glycogen Synthase antibody Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody(ab2479): 5 µg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479)
All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody (ab2479) at 1/1000 dilution
Lanes 1 & 5 : mock treated mouse cardiac myocyte lysate at 12 µl
Lane 2 : insulin (10 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 3 : insulin (100 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 4 : insulin (1 mM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 6 : CLA treated (4 nM, 45min) mouse cardiac myocyte lysate at 12 µl
Lane 7 : CLA treated (20 nM, 45min) mouse cardiac myocyte lysate
Lane 8 : CLA treated (100 nM, 45min) mouse cardiac myocyte lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
Rabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.
Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.
A 4-20% Criterion gel for SDS-PAGE was used.
