Anti-Glucocorticoid Receptor antibody [BuGR2]

• 产品名称

  Anti-Glucocorticoid Receptor抗体[BuGR2]
  参阅全部 Glucocorticoid Receptor 一抗

• 描述

  小鼠单克隆抗体[BuGR2] to Glucocorticoid Receptor

• 宿主

  Mouse

• 特异性

  Immunocytochemical staining of GR in L929 cells with this antibody results in staining of both the cytoplasm and nucleus, even in the presence of hormone. This antibody, using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.

• 经测试应用

  适用于: ICC/IF, Flow Cyt, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  不与反应: Bird, Non human primates, Amphibian

• 免疫原

  Full length protein corresponding to Rat Glucocorticoid Receptor. Partially purified rat GR.

• 阳性对照

• ICC/IF: A549, HeLa, and U251 cells; Flow Cyt: Jurkat, HeLa, and NIH/3T3 cells; WB: Mouse liver lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  BuGR2

• 同种型

  IgG2

The Abpromise guarantee

Abpromise™承诺保证使用ab2768于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 2908 Human

• Entrez Gene: 14815 Mouse

• Entrez Gene: 24413 Rat

• Omim: 138040 Human

• SwissProt: P04150 Human

• SwissProt: P06537 Mouse

• SwissProt: P06536 Rat

• Unigene: 122926 Human

• Unigene: 129481 Mouse

• Unigene: 90070 Rat

别名

• GCCR antibody

• GCR antibody

• GCR_HUMAN antibody

• GCRST antibody

• glucocorticoid nuclear receptor variant 1 antibody

• Glucocorticoid receptor antibody

• GR antibody

• GRL antibody

• Grl1 antibody

• nr3c1 antibody

• Nuclear receptor subfamily 3 group C member 1 antibody

• nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] - ChIP Grade (ab2768)

  Immunocytochemistry/Immunofluorescence analysis of Glucocorticoid Receptor shows staining in A549 cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2768 (1:100) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] - ChIP Grade (ab2768)

  Immunocytochemistry/Immunofluorescence analysis of Glucocorticoid Receptor shows staining in HeLa cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2768 (1:100) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody [BuGR2] - ChIP Grade (ab2768)

  Immunocytochemistry/Immunofluorescence analysis of Glucocorticoid Receptor shows staining in U251 cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2768 (1:100) over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

• 

  Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] - ChIP Grade (ab2768)

  Flow cytometry analysis of Glucocorticoid Receptor showing positive staining in the nucleus and cytoplasm of NIH/3T3 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2768 at 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

• 

  Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] - ChIP Grade (ab2768)

  Flow cytometry analysis of Glucocorticoid Receptor showing positive staining in the nucleus and cytoplasm of Jurkat cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and then incubated with ab2768 at 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

• 

  Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] - ChIP Grade (ab2768)

  Flow cytometry analysis of Glucocorticoid Receptor showing positive staining in the nucleus and cytoplasm of Hela cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2768 at 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

• 

  Western blot - Anti-Glucocorticoid Receptor antibody [BuGR2] - ChIP Grade (ab2768)

  Western blot of glucocorticoid receptor on mouse liver extract using ab2768. Western blot of glucocorticoid receptor on mouse liver extract using ab2768.

• 

  Flow Cytometry - Anti-Glucocorticoid Receptor antibody [BuGR2] - ChIP Grade (ab2768)

  Overlay histogram showing Jurkat cells stained with ab2768 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2768, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2 (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.