Anti-Neutrophil antibody [NIMP-R14]

• 产品名称

  Anti-Neutrophil抗体[NIMP-R14]
  参阅全部 Neutrophil 一抗

• 描述

  大鼠单克隆抗体[NIMP-R14] to Neutrophil

• 宿主

  Rat

• 经测试应用

  适用于: IHC-Frmore details

• 种属反应性

  与反应: Mouse
  

• 免疫原

  Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils

• 表位

  The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.

• 常规说明

  The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

  
  

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 0.1% BSA

• 浓度

• 50 µg 浓度为 0.1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  NIMP-R14

• 骨髓瘤

  210RCY3-Ag123

• 同种型

  IgG2b

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab2557于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 细胞定位

  Cell membrane; Lipid-anchor, GPI-anchor

• 数据库链接

• Entrez Gene: 110453 Mouse

• 别名

• Ly-6B antibody

• Ly6b antibody

• Lymphocyte antigen 6B antibody

• Lymphocyte antigen 6B Short name=Ly-6B antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)

  Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.