Anti-Apc1 (phospho S377)抗体
参阅全部 Apc1 一抗
兔多克隆抗体to Apc1 (phospho S377)
Rabbit
This antibody is specific for phosphorylated human APC1 protein at the pS377 residue. A BLAST analysis was used to suggest reactivity with this protein from human, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from mouse is expected, as this sequence show 90% homologous between human and mouse. Cross reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.
适用于: WB, IHC-Pmore details
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Immunogen affinity purified
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
多克隆
IgG
Abpromise™承诺保证使用ab12206于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/200 - 1/1000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa). | |
| IHC-P | Use at an assay dependent concentration. |
Entrez Gene: 64682 Human
Entrez Gene: 17222 Mouse
Omim: 608473 Human
SwissProt: Q9H1A4 Human
SwissProt: P53995 Mouse
Unigene: 436527 Human
Unigene: 277408 Mouse
Unigene: 449185 Mouse
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Western blot - Anti-Apc1 (phospho S377) antibody (ab12206)
All lanes : Anti-Apc1 (phospho S377) antibody (ab12206) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate from asynchoronous cells
Lane 2 : HeLa whole cell lysate from cells treated with nocodazole
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat-anti-Rabbit IgG [H&L] MX at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 215 kDa
Observed band size: 215 kDa
The blot was probed with ab12206 overnight at 4°C
While some phosphorylated APC1 is present in untreated cells, the amount of phosphorylated protein is increased arrested in mitosis (by treatment with with nocodazole).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc1 (phospho S377) antibody (ab12206)
ab12206 at 5.0 µg/ml staining pancreatic cancinoma cells at 60X. This image shows moderate positive cytoplasmic and occasional nuclear staining. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
