Anti-SUZ12抗体
参阅全部 SUZ12 一抗
兔多克隆抗体to SUZ12
Rabbit
适用于: IP, ChIP, ChIP/Chip, IHC-P, WB, RIPmore details
与反应: Mouse, Human, Common marmoset
Synthetic peptide corresponding to Human SUZ12 aa 700 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as ab12389)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab12073于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (3) | Use at an assay dependent concentration. |
| ChIP | (9) | Use at an assay dependent concentration. |
| ChIP/Chip | (1) | Use at an assay dependent concentration. |
| IHC-P | (2) | Use at an assay dependent concentration. |
| WB | (9) | Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 83 kDa). |
| RIP | (2) | Use at an assay dependent concentration. PubMed: 19571010 |
Entrez Gene: 23512 Human
Entrez Gene: 52615 Mouse
Omim: 606245 Human
SwissProt: Q15022 Human
SwissProt: Q80U70 Mouse
Unigene: 462732 Human
Unigene: 283410 Mouse
Unigene: 473315 Mouse
JJAZ1 antibody
Joined to JAZF1 protein antibody
KIAA0160 antibody
Polycomb protein SUZ12 antibody
Suppressor of zeste 12 homolog antibody
Suppressor of zeste 12 protein homolog antibody
SUZ12 antibody
SUZ12 polycomb repressive complex 2 subunit antibody
SUZ12_HUMAN antibody
ChET 9 protein antibody
CHET9 antibody
Chromatin precipitated E2F target 9 protein antibody
Western blot - Anti-SUZ12 antibody (ab12073)
All lanes : Anti-SUZ12 antibody (ab12073) at 1 µg/ml
Lane 1 : Wild-type Hela cell lysate
Lane 2 : SUZ12 knockout HeLa cell lysate
Lane 3 : Wild-type HAP1 cell lysate
Lane 4 : SUZ12 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDawhy is the actual band size different from the predicted?
Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab12073 was shown to react with SUZ12 in western blot. The band observed in the SUZ12 knockout cell line ab264983 (SUZ12 knockout cell lysate ab257721) lane below 95 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab12073 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-SUZ12 antibody (ab12073)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SUZ12 knockout HAP1 cell lysate (20 µg)
Lane 3: Caco2 cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab12073 was shown to specifically recognize SUZ12 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when SUZ12 knockout samples were used. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. ab12073 and ab8245 (loading control to GAPDH) were diluted to 1µg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-SUZ12 antibody (ab12073)
All lanes : Anti-SUZ12 antibody (ab12073)
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SUZ12 CRISPR/Cas9 edited HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDawhy is the actual band size different from the predicted?
Lanes 1- 2: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab12073 was shown to react with SUZ12 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab264983 (CRISPR/Cas9 edited cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and SUZ12 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 µg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SUZ12 antibody - ChIP Grade (ab12073)
All lanes : Anti-SUZ12 antibody (ab12073) at 1 µg/ml
Lane 1 : untransfected 293T cell lysate
Lane 2 : 293T cells transfected with 5ug HA-Suz12
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) (Dako) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDawhy is the actual band size different from the predicted?
Additional bands at: 76 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-SUZ12 antibody - ChIP Grade (ab12073)
All lanes : Anti-SUZ12 antibody (ab12073) at 1 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDawhy is the actual band size different from the predicted?
Additional bands at: 30 kDa, 53 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
