Anti-MiTF antibody [C5]

• 产品名称

  Anti-MiTF抗体[C5]
  参阅全部 MiTF 一抗

• 描述

  小鼠单克隆抗体[C5] to MiTF

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, WB, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Chicken

• 免疫原

  Recombinant fragment corresponding to Human MiTF (N terminal).

• 阳性对照

• WB: Malme-3M whole cell lysate. Flow Cyt (Intra): Malme-3M cells. ICC/IF: Malme-3M cells

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  C5

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab12039于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 4286 Human

• Entrez Gene: 17342 Mouse

• Entrez Gene: 25094 Rat

• Omim: 156845 Human

• SwissProt: O75030 Human

• SwissProt: Q08874 Mouse

• SwissProt: O88368 Rat

• Unigene: 166017 Human

• Unigene: 618266 Human

• Unigene: 333284 Mouse

• Unigene: 454504 Mouse

• Unigene: 31427 Rat

别名

• BHLHE32 antibody

• bHLHe32 antibody

• Class E basic helix-loop-helix protein 32 antibody

• CMM8 antibody

• Homolog of mouse microphthalmia antibody

• Mi antibody

• Microphthalmia associated transcription factor antibody

• Microphthalmia, mouse, homolog of antibody

• Microphthalmia-associated transcription factor antibody

• MITF antibody

• MITF_HUMAN antibody

• mitfa antibody

• nacre antibody

• WS2 antibody

• WS2A antibody

• z3A.1 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (ab12039)

  ab12039 staining MiTF in Malme-3M cells. The cells were permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab12039 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• 

  Western blot - Anti-MiTF antibody [C5] (ab12039)

  Anti-MiTF antibody [C5] (ab12039) at 1 µg/ml + Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 59 kDa
  Observed band size: 40,55,70 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 20 minutes
  

• 

  Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (ab12039)

  Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.