Anti-YB1抗体
参阅全部 YB1 一抗
兔多克隆抗体to YB1
Rabbit
From April 2025, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
适用于: ICC/IF, WBmore details
与反应: Human
预测可用于: Mouse, Rat, Xenopus laevis
Synthetic peptide corresponding to Human YB1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as ab12411)
YB1 has a predicted band size of 36kDa. According to Evdolimova (1995) YB1 migrates by SDS-PAGE at 50kDa, which may be due to post-translational modification. YB1 is primarily detectable in the cytoplasm without any clear signal in nucleoli.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab12148于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) | Use a concentration of 1 µg/ml. |
| WB | (9) | Use a concentration of 1 - 1.4 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 36 kDa). The 50 kDa band detected is consistent with the literature describing migration of YB1. |
Entrez Gene: 4904 Human
Entrez Gene: 22608 Mouse
Omim: 154030 Human
SwissProt: P67809 Human
SwissProt: P62960 Mouse
SwissProt: P21573 Xenopus laevis
SwissProt: Q00436 Xenopus laevis
Unigene: 473583 Human
Unigene: 258204 Mouse
Unigene: 110976 Rat
Unigene: 194909 Rat
Unigene: 2633 Xenopus laevis
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Western blot - Anti-YB1 antibody (ab12148)
All lanes : Anti-YB1 antibody (ab12148) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human) Whole Cell Lysate
Lane 4 : T-47D whole cell lysate (ab14899)
Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 50 kDawhy is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab12148 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody (ab12148)
ICC/IF image of ab12148 stained human HeLa cells. The cells were PFA fixed (3.7% PFA, 5 min) and incubated with the antibody (ab12148, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT™ FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Western blot - Anti-YB1 antibody (ab12148)
Anti-YB1 antibody (ab12148) at 1 µg/ml + HEK293 Whole Cell Lysate Transiently Overexpressing YB1 at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 50 kDawhy is the actual band size different from the predicted?
YB1 has a predicted band size of 36kDa based on its primary sequence (SwissProt). According to Evdolimova (1995) YB1 migrates by SDS-PAGE at 50kDa, which may be due to post-translational modification
Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody (ab12148)
ICC/IF image of ab12148 stained human HeLa cells. The cells were PFA fixed (3.7% PFA, 5 min) and incubated with the antibody (ab12148, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT™ FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Western blot - Anti-YB1 antibody (ab12148)
All lanes : Anti-YB1 antibody (ab12148) at 1.4 µg/ml
Lane 1 : HeLa Nuclear lysate
Lane 2 : HeLa Whole cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : HEK293 Whole cell lysate
Lane 6 : HeLa Nuclear lysate with YB1 peptide (ab12411) at 1 µg/ml
Lane 7 : HeLa Whole cell lysate with YB1 peptide (ab12411) at 1 µg/ml
Lane 8 : MCF-7 cell lysate with YB1 peptide (ab12411) at 1 µg/ml
Lane 9 : Jurkat whole cell lysate with YB1 peptide (ab12411) at 1 µg/ml
Lane 10 : HEK293 whole cell lysate with YB1 peptide (ab12411) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 36 kDa
Observed band size: 36,50 kDawhy is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody (ab12148)
ICC/IF image of ab12148 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12148, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
