Anti-Chromogranin B抗体
参阅全部 Chromogranin B 一抗
兔多克隆抗体to Chromogranin B
Rabbit
适用于: WB, IHC-Pmore details
与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Human Chromogranin B aa 600-700 (C terminal) conjugated to ovalbumin. Human, mouse and rat sequences are identical
Database link: P05060
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituent: Whole serum
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab12242于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | 1/2500 - 1/10000. |
| IHC-P | 1/500 - 1/5000. |
Entrez Gene: 1114 Human
Entrez Gene: 12653 Mouse
Omim: 118920 Human
SwissProt: P05060 Human
SwissProt: P16014 Mouse
Unigene: 516874 Human
Unigene: 11090 Rat
CCB peptide antibody
Cg B antibody
CgB antibody
Chgb antibody
Chromogranin-B antibody
ChromograninB antibody
SCG1 antibody
SCG1_HUMAN antibody
Secretogranin 1 antibody
Secretogranin B antibody
Secretogranin I antibody
SgI antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin B antibody (ab12242)
ab12242(1:1000) staining Chromogranin B in human pancreas (IgG inset) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Chromogranin B in secretory compartments of the pancreas.
Insert panel depicts negative control (no primary antibody).
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX.
Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
