Anti-JLP抗体
参阅全部 JLP 一抗
兔多克隆抗体to JLP
Rabbit
适用于: IHC-P, WB, IPmore details
与反应: Mouse, Human
预测可用于: Chimpanzee, Baboon, Rhesus monkey, Orangutan
Synthetic peptide corresponding to Human JLP aa 775-825.
Database link: O60271
HeLa nuclear extract.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
100 µl 浓度为 1 mg/ml
Immunogen affinity purified
This antibody is affinity purified.
多克隆
IgG
Abpromise™承诺保证使用ab12332于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 156.8 kDa. | |
| IP | Use at 6 µg/mg of lysate. |
Entrez Gene: 9043 Human
Entrez Gene: 70834 Mouse
Omim: 605430 Human
SwissProt: O60271 Human
SwissProt: Q58A65 Mouse
SwissProt: Q8CJC2 Mouse
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (ab12332)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling JLP with ab12332 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-JLP antibody (ab12332)
All lanes : Anti-JLP antibody (ab12332) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 156.8 kDa
Exposure time: 30 seconds
Western blot - Anti-JLP antibody (ab12332)
All lanes : Anti-JLP antibody (ab12332) at 0.1 µg/ml
Lane 1 : TCKM whole cell lysate at 50 µg
Lane 2 : Renca whole cell lysate at 15 µg
Lane 3 : CT26 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 156.8 kDa
Exposure time: 10 seconds
Western blot - Anti-JLP antibody (ab12332)
Samples:
Nuclear extract (NE, 20 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-JLP antibody ab12332 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Samples: Nuclear extract (NE, 20 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-JLP antibody ab12332 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation - Anti-JLP antibody (ab12332)
Immunoprecipitation analaysis of HeLa whole cell lysate (1 mg for IP; 20% of IP loaded). ab12332 was used for immunoprecipitation at 6 µg/mg lysate (lanes 1 and 2). Lane 3 - control IgG. For western blot ab12332 was used at 1 µg/ml.
