Anti-Topoisomerase II alpha抗体
参阅全部 Topoisomerase II alpha 一抗
兔多克隆抗体to Topoisomerase II alpha
Rabbit
适用于: IHC-P, IPmore details
与反应: Human
预测可用于: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide representing a portion of human DNA topoisomerase II, alpha isozyme encoded within exon 35 (LocusLink ID 7153).
HeLa cells. IHC-P: FFPE human testis tissue sections.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab12318于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at 2-10 µg/mg of lysate. |
Entrez Gene: 7153 Human
Entrez Gene: 21973 Mouse
Omim: 126430 Human
SwissProt: P11388 Human
SwissProt: Q01320 Mouse
Unigene: 156346 Human
Unigene: 4237 Mouse
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody (ab12318)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryangeal squamous cell carcinoma labelling Topoisomerase II alpha with ab12318 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-Topoisomerase II alpha antibody (ab12318)
Detection of Human Topo II Alpha by Western Blot and Immunoprecipitation.
Samples:
Whole cell lysate (25 mcg/lane for A; 0.5 mg for IP in B; 25 mcg for Input in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-Topo II alpha antibody BL983 used at the indicated concentrations for WB (A) and IP (B). Control antibody (Ctrl. in A) and antibody for WB of immunoprecipitated Topo II alpha was a monoclonal antibody to Topo II alpha from another source. Detection: Chemiluminescence with exposure times of 5 minutes (A) or 30 seconds (B).
