Anti-RPS6 (phospho S244 + S247) antibody

• 产品名称

  Anti-RPS6 (phospho S244 + S247)抗体
  参阅全部 RPS6 一抗

• 描述

  兔多克隆抗体to RPS6 (phospho S244 + S247)

• 宿主

  Rabbit

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• HeLa +/- TNF-alpha or anisomycin

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
  
  Dulbecco's PBS without magnesium and calcium

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RPS6. The final product is generated by affinity chromatography using a RPS6-derived peptide that is phosphorylated at serines 244 and 247.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab12865于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 6194 Human

• Entrez Gene: 20104 Mouse

• Entrez Gene: 667739 Mouse

• Entrez Gene: 29304 Rat

• Omim: 180460 Human

• SwissProt: P62753 Human

• SwissProt: P62754 Mouse

• SwissProt: P62755 Rat

• Unigene: 408073 Human

• Unigene: 379007 Mouse

• Unigene: 425090 Mouse

• Unigene: 34341 Rat

别名

• 40S ribosomal protein S6 antibody

• Air8 antibody

• NP33 antibody

• Phosphoprotein NP33 antibody

• Pp30 antibody

• Ribosomal protein S6 antibody

• RP S6 antibody

• rps6 antibody

• RS6 antibody

• RS6_HUMAN antibody

• S6 antibody

• S6 Ribosomal Protein antibody

• 

  Western blot - Anti-RPS6 (phospho S244 + S247) antibody (ab12865)

  Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab12865 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to RPS6 [phospho S244 & phospho S247] blocks the signal, verifying the specificity of the antibody.
  Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST