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Anti-VEGF Receptor 2 antibody

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  • 产品名称

    Anti-VEGF Receptor 2抗体
    参阅全部 VEGF Receptor 2 一抗

  • 描述

    兔多克隆抗体to VEGF Receptor 2

  • 宿主

    Rabbit

  • 特异性

    The polyclonal antibody will detect native and recombinant human VEGFR2/KDR in ELISA experiments and on the surface or solubilized from different human cell types.

  • 经测试应用

    适用于: Sandwich ELISAIPELISANeutralisingWBFlow CytIHC-Frmore details

  • 种属反应性

    与反应: Mouse, Rat, Human, Recombinant fragment

  • 免疫原

    Recombinant fragment corresponding to Human VEGF Receptor 2. Highly pure recombinant human soluble extracellular domain of KDR (110 kDa) (D1-D7).

  • 阳性对照

    • IHC: Human spleen Flow Cyt: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate and HDLEC (Human dermal lymphatic endothelial cell line) whole cell lysate

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Constituent: PBS

  • 浓度

    • 100 µg 浓度为 1 mg/ml

  • 纯度

    Protein A purified

  • 纯化说明

    This antibody is affinity purified from rabbit sera.

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab11939于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
Sandwich ELISA

Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228)

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab42228 as Capture.

Read More
IP

Use at an assay dependent concentration.

ELISA

Use at an assay dependent concentration.

Neutralising

Use at an assay dependent concentration. PubMed: 17991870

WB(1)

Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 151 kDa.

Flow Cyt

Use a concentration of 1 - 5 µg/ml. 

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr

Use at an assay dependent concentration.

数据库链接

别名

  • CD309 antibody

  • CD309 antigen antibody

  • EC 2.7.10.1 antibody

  • Fetal liver kinase 1 antibody

  • FLK-1 antibody

  • FLK1 antibody

  • FLK1, mouse, homolog of antibody

  • Kdr antibody

  • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody

  • Kinase insert domain receptor antibody

  • KRD1 antibody

  • Ly73 antibody

  • Protein tyrosine kinase receptor FLK1 antibody

  • Protein-tyrosine kinase receptor flk-1 antibody

  • soluble VEGFR2 antibody

  • Tyrosine kinase growth factor receptor antibody

  • Vascular endothelial growth factor receptor 2 antibody

  • VEGFR 2 antibody

  • VEGFR antibody

  • VEGFR-2 antibody

  • VEGFR2 antibody

  • VGFR2_HUMAN antibody

  • Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)

    Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)

    Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.

  • Western blot - Anti-VEGF Receptor 2 antibody (ab11939)

    Western blot - Anti-VEGF Receptor 2 antibody (ab11939)

    All lanes : Anti-VEGF Receptor 2 antibody (ab11939)

    Lane 1 : recombinant human soluble VEGFR-2
    Lane 2 : recombinant native VEGFR-2

    Secondary
    All lanes : anti-rabbit AP-conjugated antibody

    Predicted band size: 151 kDa



    Samples were loaded in 10% SDS-PAGE under reducing conditions.

  • Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)

    Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)

    Flow Cytometry analysis of primary human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 2 with ab11939.

  • Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)

    Flow Cytometry - Anti-VEGF Receptor 2 antibody (ab11939)

    Flow Cytometry analysis of primary human umbilical vein endothelial cells (HUVEC) labelling VEGF Receptor 2 with ab11939.

  • Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)

    Immunohistochemistry (Frozen sections) - Anti-VEGF Receptor 2 antibody (ab11939)

    Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.

  • Sandwich ELISA - Anti-VEGF Receptor 2 antibody (ab11939)

    Sandwich ELISA - Anti-VEGF Receptor 2 antibody (ab11939)

    Standard curve for VEGF Receptor 2 (Analyte: ab54347); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGF Receptor 2 (ab11939) at 0.5µg/ml.