Anti-C5a / C5a des Arg antibody [2942]

• 产品名称

  Anti-C5a / C5a des Arg抗体[2942]
  参阅全部 C5a / C5a des Arg 一抗

• 描述

  小鼠单克隆抗体[2942] to C5a / C5a des Arg

• 宿主

  Mouse

• 特异性

  This antibody reacts with a neo-epitope on C5a that is formed upon cleavage of C5 into C5a and C5b. It will detect C5a and C5a des-Arg (Not C5).

• 经测试应用

  适用于: ICC/IF, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• Eye tissue of patient with age-related macular degeneration AMD IF/ICC: HeLa cell line.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituents: PBS, 0.1% BSA

• 浓度

• 50 µg 浓度为 0.1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  0.2 µm filtered

• 克隆

  单克隆

• 克隆编号

  2942

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab11877于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 727 Human

• Omim: 120900 Human

• SwissProt: P01031 Human

• SwissProt: Q14CJ0 Human

• SwissProt: Q27I61 Human

• 别名

• Complement C5 precursor antibody

• Complement component 5 antibody

• Complement component C5a antibody

• CPAMD 4 antibody

• CPAMD4 antibody

• MGC142298 antibody

• Anaphylatoxin C5a analog antibody

• C5 antibody

• C5a anaphylatoxin antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-C5a / C5a des Arg antibody [2942] (ab11877)

  ICC/IF image of ab11877 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11877, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a / C5a des Arg antibody [2942] (ab11877)

  IHC image of ab11877 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11877, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.