Anti-Heme Oxygenase 1 antibody [HO-1-1]

• 产品名称

  Anti-Heme Oxygenase 1抗体[HO-1-1]
  参阅全部 Heme Oxygenase 1 一抗

• 描述

  小鼠单克隆抗体[HO-1-1] to Heme Oxygenase 1

• 宿主

  Mouse

• 经测试应用

  适用于: WB, Flow Cyt (Intra), Sandwich ELISA, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Dog, Human
  预测可用于: Pig

• 免疫原

  Synthetic peptide:

  MERPQPDSMPQDLSEALKEATKEVHTQAEN

  , corresponding to amino acids 1-30 of Human Heme Oxygenase 1.

  Run BLAST with Run BLAST with 

• 阳性对照

• Recombinant Human or Rat HO-1 (Hsp32) Protein. HEK293 treated with 30 uM hemin for 18hrs (see Abreview). IHC-P: FFPE human spleen normal.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: PBS, 50% Glycerol (glycerin, glycerine)

• 纯度

  Tissue culture supernatant

• 纯化说明

  Purified from TCS.

• 克隆

  单克隆

• 克隆编号

  HO-1-1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab13248于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 513221 Cow

• Entrez Gene: 442987 Dog

• Entrez Gene: 3162 Human

• Entrez Gene: 15368 Mouse

• Entrez Gene: 445512 Pig

• Entrez Gene: 24451 Rat

• Omim: 141250 Human

• SwissProt: Q5E9F2 Cow

• SwissProt: P09601 Human

• SwissProt: P14901 Mouse

• SwissProt: P32394 Pig

• SwissProt: P06762 Rat

• Unigene: 517581 Human

• Unigene: 276389 Mouse

• Unigene: 3160 Rat

别名

• 32 kD antibody

• bK286B10 antibody

• D8Wsu38e antibody

• heat shock protein 32 kD antibody

• heat shock protein 32kD antibody

• Heat shock protein antibody

• Heme oxygenase (decycling) 1 antibody

• Heme oxygenase 1 antibody

• Hemox antibody

• HMOX 1 antibody

• Hmox antibody

• Hmox1 antibody

• HMOX1_HUMAN antibody

• HO 1 antibody

• HO antibody

• HO-1 antibody

• HO1 antibody

• Hsp32 antibody

• 

  Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

  All lanes : Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1 µg/ml
  
  Lane 1 : Hek293
  Lane 2 : HL60
  Lane 3 : HeLa
  Lane 4 : A549
  Lane 5 : Hu spleen
  Lane 6 : Ms spleen
  Lane 7 : Rt spleen
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : IRDye® 800CW Goat anti Mouse
  
  Predicted band size: 34.6 kDa
  Observed band size: 32 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Hek293 & HL60 presumed negative or very low expression.

  Loading control GAPDH at 38kDa

  This image was generated using the ascites version of the product.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

  IHC image of Hem Oxygensae 1 staining in a section of formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13248, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  This image was generated using the ascites version of the product.

   

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

  The following proteins and lysates were electrophoresed; Lane 1 - Heme-Oxygenase-1 (Hsp32) Protein (50ng), lane 2 - Heme-Oxygenase-2 protein NSP-550 (100ng), lane 3 - MDBK Cell Lysate (20ug), lane 4 - Mouse liver microsome (20ug) and lane 5 - Dog liver microsome (20ug). ab13248 was applied at a concentration of 4ugml-1.

  This image was generated using the ascites version of the product.

• 

  Sandwich ELISA - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

  Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (ab85243)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5µg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (ab13243) at 0.5µg/ml.

  This image was generated using the ascites version of the product.

• 

  Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

  Lane 1 : MW marker
  Lanes 2-7 : Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)
  
  Lane 2 : Recombinant Rat Heme Oxygenase 1
  Lane 3 : Recombinant Human Heme Oxygenase 1
  Lane 4 : Recombinant Human Heme Oxygenase 2
  Lane 5 : MDBK cell lysate
  Lane 6 : Dog liver microsome
  Lane 7 : Mouse liver microsome
  
  Predicted band size: 34.6 kDa
  
  
  
  

  This image was generated using the ascites version of the product.

• 

  Western blot - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

  Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1/250 dilution + Human microsome lysate
  
  Predicted band size: 34.6 kDa
  
  
  
  

  This image was generated using the ascites version of the product.

• 

  Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

  ab13248 at 10µg/ml staining Heme Oxygenase 1 in human lung cancer A2 cells by flow cytometery. The left image repersents staining with isotype control antibody and the right image show staining with ab13248.

  This image was generated using the ascites version of the product.