Anti-Hsp20抗体
参阅全部 Hsp20 一抗
兔多克隆抗体to Hsp20
Rabbit
适用于: ICC/IF, WBmore details
与反应: Rat, Human
Rat skeletal muscle Hsp20.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituents: 0.268% PBS, 3% BSA
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab13491于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 0.05 µg/ml. Detects a band of approximately 20 kDa. |
Entrez Gene: 126393 Human
Omim: 610695 Human
SwissProt: O14558 Human
Unigene: 534538 Human
Unigene: 3201 Rat
Heat shock 20 kDa-like protein p20 antibody
Heat shock protein alpha crystallin related B6 antibody
Heat shock protein beta 6 antibody
Heat shock protein beta-6 antibody
Heat shock protein, 20-KD antibody
Heat-shock 27-KD protein 6 antibody
HEL55 antibody
Hsp20 antibody
HspB6 antibody
HSPB6_HUMAN antibody
epididymis luminal protein 55 antibody
FLJ32389 antibody
Heat shock 20 kDa like protein p20 antibody
Western blot - Anti-Hsp20 antibody (ab13491)
Anti-Hsp20 antibody (ab13491) at 1/2000 dilution + Lysates prepared from rat heart tissue
Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody (ab13491)
ICC/IF image of ab13491 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13491, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
