Anti-Hsp90抗体
参阅全部 Hsp90 一抗
兔多克隆抗体to Hsp90
Rabbit
Detects 90kD proteins corresponding to the molecular mass of hsp90aß.
适用于: ICC/IF, IHC-P, ICC, IHC-Fr, ELISA, IP, Flow Cyt, WBmore details
与反应: Mouse, Rat, Human, Xenopus laevis
Full length protein corresponding to Human Hsp90.
Database link: P08238
ICC: Heat Shocked Cervical cancer cell line (HeLa).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
浓度
100 µl 浓度为 1 mg/ml
Protein A purified
多克隆
IgG
Abpromise™承诺保证使用ab13495于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) | 1/100. |
| IHC-P | (1) | Use at an assay dependent concentration. |
| ICC | (1) | Use at an assay dependent concentration. |
| IHC-Fr | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| IP | (1) | Use at an assay dependent concentration. |
| Flow Cyt | (1) | Use at an assay dependent concentration. See Abreview. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| WB | (7) | 1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa). |
Entrez Gene: 3320 Human
Entrez Gene: 3326 Human
Entrez Gene: 15516 Mouse
Entrez Gene: 15519 Mouse
Omim: 140571 Human
Omim: 140572 Human
SwissProt: P07900 Human
SwissProt: P08238 Human
SwissProt: P07901 Mouse
SwissProt: P11499 Mouse
Unigene: 525600 Human
Unigene: 1843 Mouse
Unigene: 315997 Mouse
Unigene: 341186 Mouse
Unigene: 440626 Mouse
Unigene: 486334 Mouse
Unigene: 119867 Rat
Unigene: 232226 Rat
Unigene: 3277 Rat
Heat shock 86 kDa antibody
Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
Heat shock protein HSP 90 alpha antibody
Heat shock protein HSP 90 beta antibody
Heat shock protein HSP 90-alpha antibody
HS90A_HUMAN antibody
HSP 84 antibody
HSP 86 antibody
Hsp 90 antibody
HSP86 antibody
HSP90A antibody
HSP90AA1 antibody
HSP90AB1 antibody
HSP90B antibody
HSPC1 antibody
HSPC2 antibody
HSPCAL1 antibody
HSPCAL4 antibody
Renal carcinoma antigen NY-REN-38 antibody
Western blot - Anti-Hsp90 antibody (ab13495)
All lanes : Anti-Hsp90 antibody (ab13495) at 1/10000 dilution (2 hours at room temperature)
Lane 1 : A431 with BSA for 30 minutes at room temperature
Lane 2 : A549 with BSA for 30 minutes at room temperature
Lane 3 : HCT116 with BSA for 30 minutes at room temperature
Lane 4 : Hela with BSA for 30 minutes at room temperature
Lane 5 : HEK293 with BSA for 30 minutes at room temperature
Lane 6 : HepG2 with BSA for 30 minutes at room temperature
Lane 7 : HL-60 with BSA for 30 minutes at room temperature
Lane 8 : HUVEC with BSA for 30 minutes at room temperature
Lane 9 : Jurkat with BSA for 30 minutes at room temperature
Lane 10 : MCF7 with BSA for 30 minutes at room temperature
Lane 11 : PC3 with BSA for 30 minutes at room temperature
Lane 12 : T98G with BSA for 30 minutes at room temperature
Lysates/proteins at 2 µg per lane.
Blocking peptides at 1.5 % per lane.
Secondary
All lanes : HRP Donkey anti-rabbit IgG, 1 hour at room temperature
Predicted band size: 85 kDa
Western blot - Anti-Hsp90 antibody (ab13495)Image courtesy of Dr Mahesh Shivananjappa by Abreview.
Anti-Hsp90 antibody (ab13495) at 1/1000 dilution + whole cell lysate prepared from human platelets treated with A23187 for 1 hour at 20 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 85 kDa
Observed band size: 90 kDawhy is the actual band size different from the predicted?
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody (ab13495)
ab13495 staining Hsp90 in Mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's fixative solution. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A FITC-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)
Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked Cervical cancer cell line (HeLa) for 20min at RT, labelling Hsp90 with ab13495 at 1/100 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (green) at 1/200 dilution for 2 hours at RT. Image showing cytoplasmic staining on HeLa cells. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody (green) (C) Composite image.
