Anti-Rif1抗体
参阅全部 Rif1 一抗
兔多克隆抗体to Rif1
Rabbit
The immunogen sequence for ab13422 represents the C-terminal end of mouse Rif1, mouse teleomere-associated protein RIF1 and mouse Rap1-interacting factor.
This affinity purified antibody is directed against mouse Rif1 protein. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with Rif1 from human, chimpanzee, rat and dog as only a single amino acid residue change (92% homology) is found for the immunogen sequence. Cross reactivity with Rif1 homologues from other sources has not been determined. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 kDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 kDa) and mouse Rap1-interacting factor 1 (2426 267.1 kDa).
适用于: IP, ELISA, WBmore details
与反应: Mouse
预测可用于: Rat, Cow, Dog, Human
Synthetic peptide corresponding to Mouse Rif1 aa 2405-2419 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:
C-RNLQSRWRSPAHENS
Database link: Q6PR54
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
This affinity purified antibody is directed against mouse Rif1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Abpromise™承诺保证使用ab13422于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | ||
| ELISA | ||
| WB | (1) |
应用说明
ELISA: 1/5000 - 1/40000.
IP: Use at an assay dependent dilution (PMID 19000314).
WB: 1/500 - 1/3000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Unigene: 655671 Human
Unigene: 725538 Human
Unigene: 254530 Mouse
Entrez Gene: 55183 Human
Entrez Gene: 51869 Mouse
Omim: 608952 Human
SwissProt: Q5UIP0 Human
SwissProt: Q6PR54 Mouse
FLJ12870 antibody
Rap1 interacting factor 1 antibody
Rap1 interacting factor 1 homolog antibody
RAP1 interacting factor homolog antibody
RAP1 interacting factor homolog (yeast) antibody
Rap1-interacting factor 1 homolog antibody
Receptor interacting factor 1 antibody
RIF 1 antibody
RIF1 antibody
RIF1 isoform 4 antibody
RIF1_HUMAN antibody
Telomere associated protein RIF 1 antibody
Telomere associated protein RIF1 antibody
Telomere-associated protein RIF1 antibody
Telomeric protein RIF 1 antibody
Telomeric protein RIF1 antibody
DKFZp781N1478 antibody
FLJ10599 antibody
FLJ11269 antibody
Western blot - Anti-Rif1 antibody (ab13422)
Anti-Rif1 antibody (ab13422) at 1 µg/ml + 25 ug of MEF whole cell lysate
Secondary
IRDye TM800 conjugated goat anti rabbit IgG (H&L) incubated for 45 minutes at 1/10000 dilution
Predicted band size: 265 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
