Anti-HDAC2 antibody [HDAC2-62]

• 产品名称

  Anti-HDAC2抗体[HDAC2-62]
  参阅全部 HDAC2 一抗

• 描述

  小鼠单克隆抗体[HDAC2-62] to HDAC2

• 宿主

  Mouse

• 经测试应用

  适用于: IP, WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide:

  SGEKTDTKGTKSEQLSNP

  with N-terminally added Cysteine conjugated to KLH, corresponding to amino acids 471-488 of Human HDAC2.

  Run BLAST with Run BLAST with 

• 阳性对照

• Whole cell extracts from NIH-3T3 cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: 0.0268% PBS

• 浓度

• 批次浓度范围 50 µl 浓度为 2 - 2.4 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  HDAC2-62

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab12169于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3066 Human

• Entrez Gene: 15182 Mouse

• Omim: 605164 Human

• SwissProt: Q92769 Human

• SwissProt: P70288 Mouse

• Unigene: 3352 Human

• Unigene: 19806 Mouse

• 别名

• HDAC 2 antibody

• Hdac2 antibody

• HDAC2_HUMAN antibody

• Histone deacetylase 2 (HD2) antibody

• Histone deacetylase 2 antibody

• OTTHUMP00000017046 antibody

• OTTHUMP00000227077 antibody

• OTTHUMP00000227078 antibody

• RPD3 antibody

• transcriptional regulator homolog RPD3 antibody

• YAF1 antibody

• YY1 associated factor 1 antibody

• YY1 transcription factor binding protein antibody

• Yy1bp antibody

• D10Wsu179e antibody

• HD 2 antibody

• HD2 antibody

• 

  Immunoprecipitation - Anti-HDAC2 antibody [HDAC2-62] (ab12169)

  Western Blot using Rabbit anti-HDAC2

  
  
  Lane 1 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 5 µg
  Lane 2 : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 2.5 µg
  Lane 3 : Negative Control at 5 µg
  
  All lanes : HeLa whole cell extract
  

• 

  Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)

  All lanes : Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 0.5 µg/ml
  
  Lane 1 : Hek293T cell Lysate
  Lane 2 : HeLa cell Lysate
  Lane 3 : Jurkat cell Lysate
  Lane 4 : K562 cell Lysate
  Lane 5 : Neuro-2a cell Lysate
  Lane 6 : NIH-3T3 cell Lysate
  
  Secondary
  All lanes : Goat Anti-Mouse IgG-Peroxidase
  

• 

  Western blot - Anti-HDAC2 antibody [HDAC2-62] (ab12169)This image is courtesy of an anonymous abreview.

  Anti-HDAC2 antibody [HDAC2-62] (ab12169) at 1/250 dilution + Human glioblastoma cell line at 120 µg
  
  Secondary
  HRP-conjugated Goat anti-Mouse at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Observed band size: 55 kDawhy is the actual band size different from the predicted?
  Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 5 minutes
  

  See Abreview