Anti-Rab5抗体
参阅全部 Rab5 一抗
兔多克隆抗体to Rab5
Rabbit
适用于: IHC-P, ICC/IFmore details
与反应: Mouse, Rat, Human
预测可用于: Dog
Synthetic peptide corresponding to Human Rab5 aa 150-250 conjugated to keyhole limpet haemocyanin.
Database link: P20339
ICC/IF: Rat hippocampal neurons (embryonic day 18), HEK cells, HeLa cells. IHC-P: Mouse back skin tissue section.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituents: 2.68% PBS, 50% Glycerol (glycerin, glycerine)
浓度
100 µl 浓度为 1 mg/ml
Protein A purified
多克隆
IgG
Abpromise™承诺保证使用ab13253于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/100. Use Bouin's fixative to fix tissue. | |
| ICC/IF | 1/80. |
Entrez Gene: 5868 Human
Entrez Gene: 271457 Mouse
Omim: 179512 Human
SwissProt: P20339 Human
SwissProt: Q9CQD1 Mouse
Unigene: 475663 Human
Unigene: 329123 Mouse
RAB 5 antibody
RAB 5A antibody
RAB5A antibody
RAB5A member RAS oncogene family antibody
RAB5A_HUMAN antibody
RAS associated protein RAB5A antibody
Ras related protein Rab 5A antibody
Ras-related protein Rab-5A antibody
Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody (ab13253)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of polyclonal rabbit anti-Rab5 (Abcam ab13253, 1/200; Alexa Fluor 488; 1/200; green) and monoclonal mouse anti-tubulin-II (Alexa Fluor 568 1/200; red). Magnification 63x; insert 180x.
Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody (ab13253)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
ab13253 Rabbit polyclonal to Rab5 was used in in fixed HEK cells that had been transfected with Rab5-GFP Fluorescence microscopy of fixed Rab5-transfected HEK cells showing (left to right) immunocytochemical labelling of rabbit polyclonal Rab5 (ab13253, 1/500; Cy3; red), and Rab5-GFP (green) with their overlay (far right). Scale bar = 7µm. Showing good co-localisation.
Immunocytochemistry/ Immunofluorescence - Anti-Rab5 antibody (ab13253)
Immunofluorescent analysis of 2% paraformaldehyde fixed Human cervical cancer cell line (HeLa) for 20min at RT, labelling Rab5 with ab13253 at 1/80 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (green) at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Image showing cytoplasmic staining on HeLa cells. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody (green). (C) Composite image.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody (ab13253)
ab13253 at 1/100 dilution staining Rab5 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.
