Anti-Desmoglein 1/DSG1 antibody [27B2]

• 产品名称

  Anti-Desmoglein 1/DSG1抗体[27B2]
  参阅全部 Desmoglein 1/DSG1 一抗

• 描述

  小鼠单克隆抗体[27B2] to Desmoglein 1/DSG1

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-Frmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• IHC-Fr: Human normal skin.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  27B2

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab12077于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1828 Human

• Omim: 125670 Human

• SwissProt: Q02413 Human

• Unigene: 2633 Human

• 别名

• Desmosomal glycoprotein 1 antibody

• DG1 antibody

• DGI antibody

• DSG antibody

• DSG1 antibody

• DSG1_HUMAN antibody

• EPKHE antibody

• EPKHIA antibody

• Pemphigus foliaceus antigen antibody

• PPKS1 antibody

• SPPK1 antibody

• Cadherin family member 4 antibody

• CDHF4 antibody

• Desmoglein-1 antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)

  IHC image of ab12077 staining in 100% methanol fixed frozen tissue section of human skin.

  Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab12077 (1μg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.

  The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab12077. 

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.

   

• 

  Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)This image is courtesy of an anonymous Abreview.

  ab12077 staining Desmoglein 1/DSG1 in human skin tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 30 minutes at 25°C, then incubated with ab12077 at 5µg/ml for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-mouse polyclonal (highly adsorbed), used at a 1/1000 dilution.

  See Abreview