Anti-Synaptotagmin antibody [ASV30]

• 产品名称

  Anti-Synaptotagmin抗体[ASV30]
  参阅全部 Synaptotagmin 一抗

• 描述

  小鼠单克隆抗体[ASV30] to Synaptotagmin

• 宿主

  Mouse

• 特异性

  ab13259 is not specific to SYT2 but is a pan-synaptotagmin antibody. It does not distinguish between the Synaptotagmin family of proteins.

• 经测试应用

  适用于: WB, ICC/IF, IPmore details

• 种属反应性

  与反应: Mouse, Rat
  

• 免疫原

  Full length protein corresponding to Rat Synaptotagmin. Rat brain synaptic junction protein complexes
  Database link: P21707

• 阳性对照

• Mouse or Rat brain tissue extract.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 5th July 2019. Lot numbers higher than GR3258922 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituents: PBS, 50% Glycerol (glycerin, glycerine)

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  Purified from TCS.

• 克隆

  单克隆

• 克隆编号

  ASV30

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab13259于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 20979 Mouse

• Entrez Gene: 20980 Mouse

• Entrez Gene: 24805 Rat

• Entrez Gene: 25716 Rat

• SwissProt: P46096 Mouse

• SwissProt: P46097 Mouse

• SwissProt: P21707 Rat

• SwissProt: P29101 Rat

• Unigene: 5102 Mouse

• Unigene: 88574 Rat

别名

• HsT1192 antibody

• MYSPC antibody

• P65 antibody

• SVP65 antibody

• synaptotagmin 4 antibody

• Synaptotagmin I antibody

• Synaptotagmin II antibody

• Synaptotagmin IV antibody

• Synaptotagmin V antibody

• Synaptotagmin-2 antibody

• synaptotagmin-3 antibody

• SYT antibody

• SYT1 antibody

• Syt2 antibody

• SYT2_HUMAN antibody

• SYT3 antibody

• SYT4 antibody

• SYT5 antibody

• SytII antibody

• SytIII antibody

• sytIV antibody

• 

  Western blot - Anti-Synaptotagmin antibody [ASV30] (ab13259)

  All lanes : Anti-Synaptotagmin antibody [ASV30] (ab13259) at 1/1000 dilution
  
  Lane 1 : Molecular weight ladder
  Lane 2 : Lysates prepared from mouse brain
  Lane 3 : Lysates prepared from rat brain
  
  
  

  This image was generated using the ascites version of the product.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin antibody [ASV30] (ab13259)

  ICC/IF image of ab13259 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

  This image was generated using the ascites version of the product.

• 

  Immunoprecipitation - Anti-Synaptotagmin antibody [ASV30] (ab13259)

  Synaptotagmin was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Mouse monoclonal to Synaptotagmin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13259.
  Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
  Band: 64kDa; Synaptotagmin

  This image was generated using the ascites version of the product.