Anti-alpha Tubulin antibody [B-5-1-2]

• 产品名称

  Anti-alpha Tubulin抗体[B-5-1-2]
  参阅全部 alpha Tubulin 一抗

• 描述

  小鼠单克隆抗体[B-5-1-2] to alpha Tubulin

• 宿主

  Mouse

• 经测试应用

  适用于: ICCmore details

• 种属反应性

  与反应: Human
  预测可用于: Dog, Pig, Xenopus laevis, Cynomolgus monkey, African green monkey, Xenopus tropicalis

• 免疫原

  Full length native protein (purified) corresponding to alpha Tubulin. Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

• 表位

  The antibody recognizes an epitope located at the C-terminal end of the alpha tubulin isoform.

• 阳性对照

• 293T whole cell lysate (ab95494) can be used as a positive control in WB. Total cell extract of the Human foreskin fibroblast cell line (FS11).

• 常规说明

  Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: 0.0268% PBS

• 浓度

• 50 µl 浓度为 2.4 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  B-5-1-2

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11304于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7277 Human

• Omim: 191110 Human

• SwissProt: P68366 Human

• Unigene: 75318 Human

• 别名

• bA408E5.3 antibody

• H2 ALPHA antibody

• Hum a tub1 antibody

• Hum a tub2 antibody

• LIS3 antibody

• MGC171407 antibody

• MGC55332 antibody

• TBA4A_HUMAN antibody

• Testis-specific alpha-tubulin antibody

• TUBA1 antibody

• TUBA1A antibody

• tuba1l antibody

• Tuba4a antibody

• Tubulin alpha 1 chain antibody

• Tubulin alpha antibody

• Tubulin alpha-1 chain antibody

• tubulin alpha-1B chain antibody

• Tubulin alpha-4A chain antibody

• Tubulin H2-alpha antibody

• Tubulin, alpha 1 (testis specific) antibody

• tubulin, alpha 1, like antibody

• Tubulin, alpha 4a antibody

• Tubulin, alpha, testis-specific antibody

• Tubulin, alpha-1 antibody

• Alpha-tubulin 1 antibody

• ALS22 antibody

• B ALPHA 1 antibody

• 

  Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)

  ab11304 staining  alpha Tubulin in the HEK293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence) transfected with a recombinant protein HA tagged dual stained with Anti-HA Mouse primary antibody and Anti-Mouse Alexa Fluor®543 secondary antibody. Microtubules detected with Anti-α-tubulin Mouse primary antibody and Anti-Mouse Alexa Fluor®488 secondary antibody.Nuclus visualised with DAPI.

• 

  Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] (ab11304)This image is courtesy of an Anonymous Abreview

  ab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.

   

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