Anti-PDIA6抗体
参阅全部 PDIA6 一抗
兔多克隆抗体to PDIA6
Rabbit
适用于: ICC/IF, WBmore details
与反应: Human
预测可用于: Rat, Hamster, Xenopus laevis, Zebrafish
Synthetic peptide corresponding to Human PDIA6 aa 476-492.
Sequence:
DIDLSDVELDDLGKDEL
(Peptide available as ab11432)
WB: A549, A431, HeLa, MCF7, Caco-2, HepG2, K-562 and COLO 205 cell lysates ICC/IF: A549 and HeLa cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab11432于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/250. | |
| WB | 1/250. Detects a band of approximately 50 kDa.Can be blocked with Rabbit polyclonal to PDIA6 (ab11432). |
Entrez Gene: 10130 Human
Omim: 611099 Human
SwissProt: Q15084 Human
Unigene: 212102 Human
Unigene: 580464 Human
Unigene: 2685 Rat
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Western blot - Anti-PDIA6 antibody (ab11432)
All lanes : Anti-PDIA6 antibody (ab11432) at 1/250 dilution
Lane 1 : A549 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : K-562 cell lysate
Lane 6 : Caco-2 cell lysate
Lane 7 : HepG2 cell lysate
Lane 8 : COLO 205 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/2500 dilution
Observed band size: 48 kDawhy is the actual band size different from the predicted?
Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody (ab11432)
Immunocytochemistry/ Immunofluorescence analysis of 70% confluent log phase A549 cells labeling PDIA6 with ab11432. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab11432 at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin, 1/300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody (ab11432)
ICC/IF image of ab11432 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
