Anti-beta IV Tubulin antibody [ONS.1A6]

• 产品名称

  Anti-beta IV Tubulin抗体[ONS.1A6]
  参阅全部 beta IV Tubulin 一抗

• 描述

  小鼠单克隆抗体[ONS.1A6] to beta IV Tubulin

• 宿主

  Mouse

• 经测试应用

  适用于: ICC, IHC-P, WBmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Rat, Chicken, Hamster, Cow

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: HeLa nuclear extract and HeLa, HEK293, K562, NIH3T3 and U20S whole cell lysates. ICC: MCF7 cells IHC-P: Human normal skin tissue.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  ONS.1A6

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab11315于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 540236 Cow

• Entrez Gene: 10382 Human

• Entrez Gene: 22153 Mouse

• Entrez Gene: 29213 Rat

• Omim: 602662 Human

• SwissProt: Q3ZBU7 Cow

• SwissProt: P04350 Human

• SwissProt: Q9D6F9 Mouse

• Unigene: 110837 Human

• Unigene: 7420 Mouse

别名

• Beta 4 antibody

• Beta 4 tubulin antibody

• beta 5 antibody

• beta four tubulin antibody

• Dystonia 4 torsion (autosomal dominant) antibody

• MC1R antibody

• TBB4_HUMAN antibody

• TUB B4 antibody

• TUBB 4 antibody

• tubb4 antibody

• TUBB4A antibody

• TUBB5 antibody

• Tubulin 5 beta antibody

• Tubulin beta 3 antibody

• Tubulin beta 4 antibody

• Tubulin beta 4 chain antibody

• Tubulin beta 4A class IVa antibody

• Tubulin beta 5 antibody

• Tubulin beta IV antibody

• Tubulin beta-4 chain antibody

• 

  Immunocytochemistry - Anti-beta IV Tubulin antibody [ONS.1A6] (ab11315)

  ab11315 staining Beta IV tubulin in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11315 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• 

  Western blot - Anti-beta IV Tubulin antibody [ONS.1A6] (ab11315)

  All lanes : Anti-beta IV Tubulin antibody [ONS.1A6] (ab11315) at 5 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
  Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
  Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
  Lane 6 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 50 kDa
  Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 4 minutes
  
  
  

  This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab11315 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [ONS.1A6] (ab11315)

  IHC image of beta IV Tubulin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11315, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunocytochemistry - Anti-beta IV Tubulin antibody [ONS.1A6] (ab11315)This image is courtesy of Xinmei Chen, University of Alberta

  Immunofluorescence analysis of MCF-7 Cells (human breast cancer cell line), staining beta IV Tubulin with ab11315.
  
  Cells on coverslip were fixed with -20°C Methanol for 5 min before permeabilization with 0.2% Triton X-100 in PBS for 10 min. Cells were incubated with the primary antibody with a dilution 1/50 for 1 hour. After brief washing with PBS, cells were incubated with TRITC conjugated secondary antibody with a dilution 1/50 for 45 min and washed with PBS to be examined under fluorescence microscope.

  See Abreview

• 

  Immunocytochemistry - Anti-beta IV Tubulin antibody [ONS.1A6] (ab11315)This image is courtesy of Xinmei Chen, University of Alberta

  Immunofluorescence analysis of MCF-7 Cells (human breast cancer cell line), staining beta IV Tubulin with ab11315.
  
  Cells on coverslip were fixed with -20°C Methanol for 5 min before permeabilization with 0.2% Triton X-100 in PBS for 10 min. Cells were incubated with the primary antibody with a dilution 1/50 for 1 hour. After brief washing with PBS, cells were incubated with TRITC conjugated secondary antibody with a dilution 1/50 for 45 min and washed with PBS to be examined under fluorescence microscope.