Anti-Histone H2A.X抗体
参阅全部 Histone H2A.X 一抗
兔多克隆抗体to Histone H2A.X
Rabbit
适用于: WB, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rabbit, Rhesus monkey, Gorilla
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
IHC: Human ovarian carcinoma tissue WB: HEK293T, L1210 and F9 cell lysates
The phosphorylated form of this Ab is known as gamma H2A.X, when phosphorylated at Ser 139.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.1% Sodium azide
Constituent: Tris citrate/phosphate
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab11175于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (23) | Use a concentration of 0.1 µg/ml. Detects a band of approximately 15 kDa. |
| IHC-P | (2) | 1/1000. |
Entrez Gene: 3014 Human
Entrez Gene: 15270 Mouse
Omim: 601772 Human
SwissProt: P16104 Human
SwissProt: P27661 Mouse
Unigene: 477879 Human
Unigene: 245931 Mouse
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Western blot - Anti-Histone H2A.X antibody (ab11175)
Anti-Histone H2A.X antibody (ab11175) at 0.1 µg/ml + HEK293T cells at 50 µg
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.X antibody (ab11175)
mmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling H2A.X with ab11175 at 1 µg/mL.
Western blot - Anti-Histone H2A.X antibody (ab11175)
All lanes : Anti-Histone H2A.X antibody (ab11175) at 0.1 µg/ml
Lane 1 : F9 cell lysate (prepared using RIPA lysis buffer)
Lane 2 : L1210 cell lysate (prepared using RIPA lysis buffer)
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
Detection: Chemiluminescence
