Anti-Syntrophin antibody [1351]

• 产品名称

  Anti-Syntrophin抗体[1351]

• 描述

  小鼠单克隆抗体[1351] to Syntrophin

• 宿主

  Mouse

• 特异性

  ab11425 is known to be reactive with the alpha 1, beta 1 and beta 2 subunits of syntrophin.

• 经测试应用

  适用于: ICC/IF, IP, WB, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  预测可用于: Fish

• 免疫原

  Full length native protein (purified) corresponding to Syntrophin. Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.

• 表位

  ab11425 is directed against an epitope within the PDZ domain of syntrophin.

• 阳性对照

• WB: U-87MG, PC-3, and HeLa cell lysates.

• 常规说明

  ab11425 is seen as the "gold standard" for syntrophin assessment.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: PBS, 1% BSA

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• Primary antibody说明

  ab11425 is seen as the "gold standard" for syntrophin assessment.

• 克隆

  单克隆

• 克隆编号

  1351

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11425于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 6640 Human

• Entrez Gene: 6641 Human

• Entrez Gene: 6645 Human

• SwissProt: Q13424 Human

• SwissProt: Q13425 Human

• SwissProt: Q13884 Human

• SwissProt: Q61234 Mouse

• Unigene: 31121 Human

• Unigene: 1541 Mouse

别名

• 59 kDa dystrophin-associated protein A1 acidic component 1 antibody

• Alpha-1-syntrophin antibody

• Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody

• SNT1 antibody

• SNT2 antibody

• SNT3 antibody

• Snta1 antibody

• SNTA1_HUMAN antibody

• SNTB1 antibody

• SNTB2 antibody

• Syntrophin alpha 1 antibody

• Syntrophin beta 1 antibody

• Syntrophin beta 2 antibody

• Syntrophin-1 antibody

• TACIP1 antibody

• 

  Western blot - Anti-Syntrophin antibody [1351] (ab11425)

  All lanes : Anti-Syntrophin antibody [1351] (ab11425) at 1 µg/ml
  
  Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
  Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
  Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG (H+L) (HRP) at 1/4000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 54 kDa
  Observed band size: 54 kDa
  
  
  
  

  Protein samples were electrophoresed by SDS-PAGE using a 12% Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane.The membrane was probed with the relevant primary and secondary antibodies following blocking with 5% skimmed milk.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin antibody [1351] (ab11425)

  Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Syntrophin (green) with ab11425 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

• 

  Flow Cytometry - Anti-Syntrophin antibody [1351] (ab11425)

  Overlay histogram showing SH-SY5Y cells stained with ab11425 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11425, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Western blot - Anti-Syntrophin antibody [1351] (ab11425)

  All lanes : Anti-Syntrophin antibody [1351] (ab11425) at 5 µg/ml
  
  Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
  Lane 2 : Skeletal Muscle (Rat) Tissue Lysate
  Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 54 kDa
  Observed band size: 58 kDawhy is the actual band size different from the predicted?
  Additional bands at: 26 kDa, 42 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 12 minutes
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin antibody [1351] (ab11425)

  Immunocytochemistry/Immunofluorescence analysis of A2058 cells labeling Syntrophin (green) with ab11425 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin antibody [1351] (ab11425)

  Immunocytochemistry/Immunofluorescence analysis of 293 cells labeling Syntrophin (green) with ab11425 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

• 

  Immunoprecipitation - Anti-Syntrophin antibody [1351] (ab11425)

  Syntrophin was immunoprecipitated using 0.5mg Mouse Skeletal Muscle tissue lysate, 5µg of Mouse monoclonal to Syntrophin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Mouse Skeletal Muscle tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab11425.
  Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
  Band: 58kDa; Syntrophin