Anti-Sumo 1抗体
参阅全部 Sumo 1 一抗
兔多克隆抗体to Sumo 1
Rabbit
适用于: IHC-P, IPmore details
与反应: Human
Recombinant full length protein corresponding to Human Sumo 1.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab11672于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IP | (1) | Use at an assay dependent concentration. |
Entrez Gene: 7341 Human
Omim: 601912 Human
SwissProt: P63165 Human
Unigene: 596171 Human
Unigene: 81424 Human
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Immunoprecipitation - Anti-Sumo 1 antibody (ab11672)
293T cells were transfected with a vector that has Sumo1 fused to GFP and a Flag tag. Cell lysates were used in IP with ab11672 (and a GFP antibody as a control). The resulting western blot was performed with a Flag antibody. As a control, cells were transfected with a a vector with Sumo2 fused to GFP and a Flag tag. ab11672 does not IP anything from this lysate.
Lane 1: Sumo1 fusion lysate - IP'd with GFP antibody
Lane 2: Sumo1 fusion lysate - no IP
Lane 3: Sumo1 fusion lysate - IP'd with ab11672
Lane 4: Sumo2 fusion lysate - IP'd with ab11672
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody (ab11672)
Ab11672 staining human normal placenta. Staining is localized to the nucleus and nuclear membrane.
Left panel: with primary whole serum antibody at 1/400. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be
