Anti-Laminin 2 alpha antibody [4H8-2]

• 产品名称

  Anti-Laminin 2 alpha抗体[4H8-2]
  参阅全部 Laminin 2 alpha 一抗

• 描述

  大鼠单克隆抗体[4H8-2] to Laminin 2 alpha

• 宿主

  Rat

• 经测试应用

  适用于: IHC-Frmore details

• 种属反应性

  与反应: Mouse
  预测可用于: Human

• 免疫原

  Full length protein corresponding to Mouse Laminin 2 alpha. Heart Laminin 2 (Mouse).

• 表位

  The epitope recognized by the antibody resides in the N-terminal portion of the alpha 2 chain of laminin.

• 阳性对照

• IHC-Fr: Mouse tongue tissue.

• 常规说明

  Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: 0.0268% PBS

• 浓度

• 25 µl 浓度为 2.4 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  4H8-2

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11576于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3908 Human

• Entrez Gene: 16773 Mouse

• Omim: 156225 Human

• SwissProt: P24043 Human

• SwissProt: Q60675 Mouse

• Unigene: 200841 Human

• Unigene: 256087 Mouse

• 别名

• Laminin alpha 2 (merosin congenital muscular dystrophy) antibody

• Laminin alpha 2 antibody

• Laminin alpha 2 chain antibody

• Laminin alpha 2 subunit antibody

• Laminin M antibody

• Laminin M chain antibody

• Laminin subunit alpha-2 antibody

• Laminin-12 subunit alpha antibody

• Laminin-2 subunit alpha antibody

• Laminin-4 subunit alpha antibody

• LAMM antibody

• Merosin heavy chain antibody

• LAMA 2 antibody

• LAMA2 antibody

• LAMA2_HUMAN antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)

  Immunohistochemical analysis of acetone-fixed frozen section of mouse tongue tissue labeling Laminin 2 alpha using ab11576 at 8 μg/mL, followed by a monoclonal Anti-Rat Kappa & Lamda light chains-FITC congugate at 1/40 dilution (Green).

• 

  Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)This image is courtesy of an anonymous Abreview.

  Immunohistochemistry (Frozen sections) analysis of Mouse muscle tissue labeling Laminin 2 alpha with ab11576 at 1/200 dilution. Tissue fixed with paraformaldehyde and permeabilized with 0.3% Triton X-100 in PBS for 30 minutes. Tissue blocked with 10% goat serum for 1 hour at room temperature followed by incubation with the primary antibody in 2% goat serum, 0.2% triton X-100 in PBS overnight at 4°C. Goat anti-rat IgG (Alexa Fluor® 568) was used as the secondary antibody at 1/500 dilution (red).

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• 

  Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)This image is courtesy of an Abreview submitted by Dr Ling Guo

  ab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr.  The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours.  A rhodamine-conjugated goat anti-rat antibody was used as the secondary.

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