Anti-TTK/Mps1 antibody [N1] - BSA free

• 产品名称

  Anti-TTK/Mps1抗体[N1] - BSA free
  参阅全部 TTK/Mps1 一抗

• 描述

  小鼠单克隆抗体[N1] to TTK/Mps1 - BSA free

• 宿主

  Mouse

• 经测试应用

  适用于: WBmore details
  不适用于: Flow Cyt or ICC/IF

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant fragment within Human TTK/Mps1 aa 1-900. The exact sequence is proprietary. (catalytically inactive - D663A)

• 表位

  This antibody recognizes an epitope in the N-terminal region of Mps1.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  N1

• 骨髓瘤

  PAIB3Ag81

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11108于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for Flow Cyt or ICC/IF.

• 数据库链接

• Entrez Gene: 7272 Human

• Omim: 604092 Human

• SwissProt: P33981 Human

• Unigene: 169840 Human

• 别名

• ECSTY kinase antibody

• ESK antibody

• FLJ38280 antibody

• hMPS1 antibody

• Monopolar Spindle 1 Like 1 antibody

• MPH1 antibody

• Mps 1 antibody

• MPS1L1 antibody

• Phosphotyrosine Picked Threonine Kinase antibody

• Phosphotyrosine picked threonine-protein kinase antibody

• PYT antibody

• RP3-357D13.2 antibody

• ttk antibody

• TTK Protein Kinase antibody

• TTK_HUMAN antibody

• cancer/testis antigen 96 antibody

• CT96 antibody

• Dual specificity protein kinase TTK antibody

• 

  Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)

  All lanes : Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
  Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 4 : Jurkat nuclear extract lysate (ab14844)
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 95 kDa
  Observed band size: 100 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 8 minutes
  
  
  NOTE: MILK was used to block non-specific binding, rather than BSA.

• 

  Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108)

  Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 10 µg/ml + Recombinant human TTK/Mps1 protein (ab89589) at 0.01 µg
  
  Secondary
  Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 95 kDa
  
  
  Exposure time: 8 minutes
  
  
  

  ab11108 recognizes the full length tagged recombinant TTK/Mps1 protein (ab89589) which has an expected molecular weight of 130 kDa.