Anti-S1P1/EDG1抗体
参阅全部 S1P1/EDG1 一抗
兔多克隆抗体to S1P1/EDG1
Rabbit
ab11424 detects S1P1/EDG1 protein in transfected human cell samples. This antibody shows no cross-reactivity to S1P3.
适用于: IP, ICC/IF, IHC-P, WB, Flow Cyt, ICCmore details
与反应: Mouse, Rat, Human
预测可用于: Horse, Cow, Cat, Monkey
Synthetic peptide corresponding to Mouse S1P1/EDG1 aa 359-372.
Sequence:
SHPQKDDGDNPETI
(Peptide available as ab39763)
WB: Rat brain tissue lysate; Neuro-2a cell lysate. ICC: CCL39 and Neuro-2a cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab11424于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | (3) | Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa. Band represents recombinant S1P1 from transfected HEK293 cells. |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 20733200 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. | |
| ICC | Use a concentration of 2 µg/ml. |
Entrez Gene: 100050049 Horse
Entrez Gene: 1901 Human
Entrez Gene: 13609 Mouse
Omim: 601974 Human
SwissProt: P21453 Human
SwissProt: O08530 Mouse
Unigene: 154210 Human
Unigene: 982 Mouse
Unigene: 109455 Rat
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Western blot - Anti-S1P1/EDG1 antibody (ab11424)
Lane 1 : Anti-S1P1/EDG1 antibody (ab11424) at 2 µg/ml
Lane 2 : Anti-S1P1/EDG1 antibody (ab11424) at 2 mg/ml
Lane 1 : Rat brain tissue extract
Lane 2 : Neuro-2a Cells
Secondary
All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 0.4 µg/ml
Developed using the ECL technique.
Immunocytochemistry - Anti-S1P1/EDG1 antibody (ab11424)
Immunocytochemical analysis of Neuro-2a cells, labeling S1P1/EDG1 with ab11424. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Immunostaining with anti-EDG1 diluted 2µg/ml in 0.1% BSA for 3 hours at room temperature.
Green staining - ab11424
Blue staining - DAPI for nuclear localization
Red staining - F-actin
The right-most image is a control (no primary antibody staining).
Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody (ab11424)
ab11424 staining S1P1/EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.
